Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12327 | 5' | -58.7 | NC_003324.1 | + | 50412 | 0.7 | 0.288806 |
Target: 5'- --gAGCAUAGGCaGCGGCgaucaGGCGAUCGUc -3' miRNA: 3'- aagUCGUGUCCG-CGUUG-----CCGCUGGCA- -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 52947 | 0.67 | 0.411371 |
Target: 5'- cUCAuGCGCuugagcuuaucgaAGuCGUAGCGGCGGCCGUu -3' miRNA: 3'- aAGU-CGUG-------------UCcGCGUUGCCGCUGGCA- -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 53184 | 0.68 | 0.403105 |
Target: 5'- -aCAGCAUgugguGGGUgGUAGCuGGCGGCCGg -3' miRNA: 3'- aaGUCGUG-----UCCG-CGUUG-CCGCUGGCa -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 53415 | 0.69 | 0.326476 |
Target: 5'- gUCugcGCACGGaugaaguccauGCGCucAACGGCGACCGa -3' miRNA: 3'- aAGu--CGUGUC-----------CGCG--UUGCCGCUGGCa -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 57380 | 0.73 | 0.191151 |
Target: 5'- cUCGGCGgAGGCGagGGCGGCacGACCGg -3' miRNA: 3'- aAGUCGUgUCCGCg-UUGCCG--CUGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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