Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12327 | 5' | -58.7 | NC_003324.1 | + | 36528 | 0.67 | 0.46012 |
Target: 5'- --aAGCGCcgcGGGCGCGACGGCauGCCc- -3' miRNA: 3'- aagUCGUG---UCCGCGUUGCCGc-UGGca -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 30378 | 0.67 | 0.449343 |
Target: 5'- gUCGcUACAGGCauguucgGCGGCuugGGCGACCGUg -3' miRNA: 3'- aAGUcGUGUCCG-------CGUUG---CCGCUGGCA- -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 27461 | 0.67 | 0.440629 |
Target: 5'- cUCAGCGCAGGCcuguaGACGGCacgaaguACCGc -3' miRNA: 3'- aAGUCGUGUCCGcg---UUGCCGc------UGGCa -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 24166 | 0.67 | 0.431061 |
Target: 5'- aUCAGCGCcaccguGGCGCAGC-GC-ACCGg -3' miRNA: 3'- aAGUCGUGu-----CCGCGUUGcCGcUGGCa -5' |
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12327 | 5' | -58.7 | NC_003324.1 | + | 30315 | 0.66 | 0.510728 |
Target: 5'- gUCGGCGguGGCGUcgcuCGGCGcguGCUGg -3' miRNA: 3'- aAGUCGUguCCGCGuu--GCCGC---UGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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