miRNA: 3'- aagUCGUgUCCgCGUUGCCGcUGGCA- -5'
miRNA: 3'- aaGUCGUGUCCgcGUuGCCgCUGGCA- -5'
miRNA: 3'- aaGUCGUGUCCG---------CGUugCCGCUGGCA- -5'
miRNA: 3'- aaGUCGUGUCCg----CG-------------UugCCGCUGGCa -5'
miRNA: 3'- aaGUCGUGUCCGCguU---GCCGCUGGCa -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12327 | 5' | -58.7 | NC_003324.1 | + | 3269 | 0.66 | 0.480059 |
|
Target: 5'- --aAGCGgAGG-GCGGCGGCaACCGUc -3' miRNA: 3'- aagUCGUgUCCgCGUUGCCGcUGGCA- -5' |
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| 12327 | 5' | -58.7 | NC_003324.1 | + | 24592 | 0.66 | 0.470036 |
|
Target: 5'- -cCAGCGCAGGauCAuCGG-GGCCGUa -3' miRNA: 3'- aaGUCGUGUCCgcGUuGCCgCUGGCA- -5' |
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| 12327 | 5' | -58.7 | NC_003324.1 | + | 41561 | 0.67 | 0.46012 |
|
Target: 5'- -gCGGCGCGGGCcuguuccucGCA--GGUGGCCGUc -3' miRNA: 3'- aaGUCGUGUCCG---------CGUugCCGCUGGCA- -5' |
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| 12327 | 5' | -58.7 | NC_003324.1 | + | 37454 | 0.67 | 0.439667 |
|
Target: 5'- -aCAGCGCGGGauggaGCcggggucuccgggAugGGCGACUGg -3' miRNA: 3'- aaGUCGUGUCCg----CG-------------UugCCGCUGGCa -5' |
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| 12327 | 5' | -58.7 | NC_003324.1 | + | 14601 | 0.78 | 0.076697 |
|
Target: 5'- -gCGGCGCAGGCGguAgcgUGGCGGCCGg -3' miRNA: 3'- aaGUCGUGUCCGCguU---GCCGCUGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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