Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12328 | 3' | -64 | NC_003324.1 | + | 16214 | 0.68 | 0.243947 |
Target: 5'- gCGGCCCC-CGCuaccgaagCGCCCuGCGCa--GGa -3' miRNA: 3'- -GCCGGGGuGCG--------GCGGGuCGCGcaaCC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 30019 | 0.68 | 0.243947 |
Target: 5'- -cGCCCCuCGcCCGUCCGGaC-CGUUGGg -3' miRNA: 3'- gcCGGGGuGC-GGCGGGUC-GcGCAACC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 43654 | 0.68 | 0.24992 |
Target: 5'- aGGCCCUcaaucggcugACGuuGCgCGGCGUG-UGGc -3' miRNA: 3'- gCCGGGG----------UGCggCGgGUCGCGCaACC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 55122 | 0.68 | 0.256013 |
Target: 5'- gCGGCagCCCAgccguccgccuUGCCuGCCCGGCGCGa--- -3' miRNA: 3'- -GCCG--GGGU-----------GCGG-CGGGUCGCGCaacc -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 41629 | 0.67 | 0.262228 |
Target: 5'- aGG-CCCGCGCCGCUgucguUAGCGUuuggcacgcguuGUUGGc -3' miRNA: 3'- gCCgGGGUGCGGCGG-----GUCGCG------------CAACC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 32277 | 0.67 | 0.275024 |
Target: 5'- gGGCCUCgaagAUGCCGCgCAcgacGCGCGUcGGc -3' miRNA: 3'- gCCGGGG----UGCGGCGgGU----CGCGCAaCC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 50517 | 0.67 | 0.288315 |
Target: 5'- -aGCCCUGCGagcugcaccaCGCCCAGCGCcUUGcGg -3' miRNA: 3'- gcCGGGGUGCg---------GCGGGUCGCGcAAC-C- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 27884 | 0.66 | 0.309189 |
Target: 5'- gGGCUCgGCGCCaucaGUCCGaCGaCGUUGGg -3' miRNA: 3'- gCCGGGgUGCGG----CGGGUcGC-GCAACC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 32381 | 0.66 | 0.316399 |
Target: 5'- gCGGCgCCGCgcagagGCCGUugucgCCAGCGUGUucucUGGc -3' miRNA: 3'- -GCCGgGGUG------CGGCG-----GGUCGCGCA----ACC- -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 22013 | 0.66 | 0.34572 |
Target: 5'- uGGCCCgCAUccacaagaaguggGaaGCCCAGCGCGg--- -3' miRNA: 3'- gCCGGG-GUG-------------CggCGGGUCGCGCaacc -5' |
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12328 | 3' | -64 | NC_003324.1 | + | 9920 | 0.66 | 0.346497 |
Target: 5'- aCGGUUCCuGCGCCGCUCAuucCGUuUUGGa -3' miRNA: 3'- -GCCGGGG-UGCGGCGGGUc--GCGcAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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