Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12333 | 3' | -56.4 | NC_003324.1 | + | 32086 | 0.66 | 0.67408 |
Target: 5'- --cGGCCUCgcugcgccucggcucGUCGCCGGUgAGgCCGa -3' miRNA: 3'- gaaCCGGAG---------------UAGUGGUCGgUCaGGCg -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 31779 | 0.66 | 0.669725 |
Target: 5'- -gUGGUUcgCGUCACCAGCaCGGggaUGCg -3' miRNA: 3'- gaACCGGa-GUAGUGGUCG-GUCag-GCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 42867 | 0.66 | 0.658814 |
Target: 5'- --aGGCU-----GCCGGCCGGcCCGCg -3' miRNA: 3'- gaaCCGGaguagUGGUCGGUCaGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 28626 | 0.66 | 0.658814 |
Target: 5'- -gUGGCCcggaUCGUUGCgGGCCGccuuGUCgCGCg -3' miRNA: 3'- gaACCGG----AGUAGUGgUCGGU----CAG-GCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 33346 | 0.66 | 0.647879 |
Target: 5'- -aUGGUCaUCGUC-CCGgugcgugguuGCCGGcUCCGCg -3' miRNA: 3'- gaACCGG-AGUAGuGGU----------CGGUC-AGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 42117 | 0.66 | 0.647879 |
Target: 5'- gCUUcGCaCUCGUCGCCAauGCCg--CCGCg -3' miRNA: 3'- -GAAcCG-GAGUAGUGGU--CGGucaGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 20892 | 0.66 | 0.63693 |
Target: 5'- --cGGCgaugcgCGUCACCAaCaCGGUCCGCc -3' miRNA: 3'- gaaCCGga----GUAGUGGUcG-GUCAGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 45940 | 0.66 | 0.625976 |
Target: 5'- --cGGCCUCG-CAUCAGgCGccCCGCg -3' miRNA: 3'- gaaCCGGAGUaGUGGUCgGUcaGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 6009 | 0.66 | 0.625976 |
Target: 5'- ---aGCCgcaAUUGCCGcCCAGUCCGCu -3' miRNA: 3'- gaacCGGag-UAGUGGUcGGUCAGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 43909 | 0.66 | 0.625976 |
Target: 5'- gUUGaGCCUUuggCGCCGGCaccgAGcCCGCa -3' miRNA: 3'- gAAC-CGGAGua-GUGGUCGg---UCaGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 18100 | 0.67 | 0.615028 |
Target: 5'- -aUGGCC-CGUggCGCCGGCCAaUCgGUa -3' miRNA: 3'- gaACCGGaGUA--GUGGUCGGUcAGgCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 31251 | 0.67 | 0.615028 |
Target: 5'- --gGGCCUgAUgGCgcaGGCCGGgcagcUCCGCg -3' miRNA: 3'- gaaCCGGAgUAgUGg--UCGGUC-----AGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 3045 | 0.67 | 0.615028 |
Target: 5'- --gGGCCUguUCGCgGGCgCGGUcucucucaauggCCGCa -3' miRNA: 3'- gaaCCGGAguAGUGgUCG-GUCA------------GGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 29581 | 0.67 | 0.615028 |
Target: 5'- --aGGCCUCGUCGCUcgauuccucgGGCagaacGUCCuGCa -3' miRNA: 3'- gaaCCGGAGUAGUGG----------UCGgu---CAGG-CG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 11117 | 0.67 | 0.613933 |
Target: 5'- -aUGGCCUg--CGCUauGGCCcgaccuuGGUCCGCu -3' miRNA: 3'- gaACCGGAguaGUGG--UCGG-------UCAGGCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 11218 | 0.67 | 0.604094 |
Target: 5'- -aUGGCCUaCAUCGCCGGaaauggagaUCAG-CCGg -3' miRNA: 3'- gaACCGGA-GUAGUGGUC---------GGUCaGGCg -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 54173 | 0.67 | 0.588829 |
Target: 5'- -aUGcGCUUCGUCACaaacuggaugucgAGCCGGUCgGCc -3' miRNA: 3'- gaAC-CGGAGUAGUGg------------UCGGUCAGgCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 2492 | 0.67 | 0.582307 |
Target: 5'- --cGGCUUCAUCAUCGGC--GUUgGCg -3' miRNA: 3'- gaaCCGGAGUAGUGGUCGguCAGgCG- -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 19434 | 0.68 | 0.549954 |
Target: 5'- cCUUGGCCUUGUCcuuACCGGCaaagCCGa -3' miRNA: 3'- -GAACCGGAGUAG---UGGUCGgucaGGCg -5' |
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12333 | 3' | -56.4 | NC_003324.1 | + | 14568 | 0.68 | 0.528693 |
Target: 5'- gUUGGCUg---CACCGGCU--UCCGCg -3' miRNA: 3'- gAACCGGaguaGUGGUCGGucAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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