Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12338 | 5' | -57.6 | NC_003324.1 | + | 46962 | 0.66 | 0.57316 |
Target: 5'- --aGCCAAauucuucuaauuGGCUGaGGGCGGCGAUaacGCc -3' miRNA: 3'- gagUGGUU------------CCGGC-CCCGUCGCUAa--CG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 1868 | 0.67 | 0.5307 |
Target: 5'- gCUCGCUcacGGGCCGGGGUc-CGuucgUGCu -3' miRNA: 3'- -GAGUGGu--UCCGGCCCCGucGCua--ACG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 50018 | 0.67 | 0.520257 |
Target: 5'- aUCGgCAAGcGCUGGGGCcGCuGAUcgGCu -3' miRNA: 3'- gAGUgGUUC-CGGCCCCGuCG-CUAa-CG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 15956 | 0.67 | 0.509894 |
Target: 5'- aUCGaCAAGGCgGGGGCguuGGCGAcgauaagaUGCg -3' miRNA: 3'- gAGUgGUUCCGgCCCCG---UCGCUa-------ACG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 40258 | 0.68 | 0.489438 |
Target: 5'- -cCGCCuugcgugcGGuCCGGGGCuuGGCGggUGCc -3' miRNA: 3'- gaGUGGuu------CC-GGCCCCG--UCGCuaACG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 53817 | 0.68 | 0.489438 |
Target: 5'- -gCACCAAGGUCucgccGGGCuGCGAcaacUGCu -3' miRNA: 3'- gaGUGGUUCCGGc----CCCGuCGCUa---ACG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 1146 | 0.68 | 0.479355 |
Target: 5'- uCUCGCUGAcGCuCGGGGCgAGCGGgaaGCc -3' miRNA: 3'- -GAGUGGUUcCG-GCCCCG-UCGCUaa-CG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 1517 | 0.68 | 0.469374 |
Target: 5'- -cCAgCGAGGauCCGGcGGCAGUGG-UGCg -3' miRNA: 3'- gaGUgGUUCC--GGCC-CCGUCGCUaACG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 48817 | 0.69 | 0.402724 |
Target: 5'- -cCACCGAGGCCaaugGGGGCGaCGAggUGa -3' miRNA: 3'- gaGUGGUUCCGG----CCCCGUcGCUa-ACg -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 9552 | 0.69 | 0.393701 |
Target: 5'- gUCAgCCGgaguGGGuCUGGGGUGGCGAUgccGCa -3' miRNA: 3'- gAGU-GGU----UCC-GGCCCCGUCGCUAa--CG- -5' |
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12338 | 5' | -57.6 | NC_003324.1 | + | 44895 | 0.72 | 0.268012 |
Target: 5'- -aCGCCGGGGCgCGGGGgCuG-GAUUGCu -3' miRNA: 3'- gaGUGGUUCCG-GCCCC-GuCgCUAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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