Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12345 | 5' | -61.5 | NC_003324.1 | + | 36751 | 0.69 | 0.289534 |
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Target: 5'- cCGAUGCGaggcCCGUGGagcgcuGGCCGguguuuucgagcUCCGGCUc -3' miRNA: 3'- -GCUACGC----GGCACCg-----CCGGC------------AGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 42840 | 0.67 | 0.38438 |
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Target: 5'- uCGcgGaCGCCGUauugcugguauucgaGGCuGCCGgCCGGCCc -3' miRNA: 3'- -GCuaC-GCGGCA---------------CCGcCGGCaGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 44408 | 0.7 | 0.256208 |
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Target: 5'- ---cGCGCCGUGGCugcagaggcuaucGGCCGcgCC-ACCg -3' miRNA: 3'- gcuaCGCGGCACCG-------------CCGGCa-GGcUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 44595 | 0.68 | 0.317983 |
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Target: 5'- aGAcGCaCCGcaUGcGCGuGCCGUCCGAUCg -3' miRNA: 3'- gCUaCGcGGC--AC-CGC-CGGCAGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 48710 | 0.66 | 0.461218 |
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Target: 5'- uGAUGUugcggcggaGCCGgauGCGGgCG-CCGACCu -3' miRNA: 3'- gCUACG---------CGGCac-CGCCgGCaGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 52673 | 0.69 | 0.310679 |
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Target: 5'- cCGcAUGCGCgagCGUGGaUGGUCGaUCCGGCg -3' miRNA: 3'- -GC-UACGCG---GCACC-GCCGGC-AGGCUGg -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 54798 | 0.66 | 0.415516 |
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Target: 5'- aGAUGCGCCGau-CGaG-CGUUCGACCg -3' miRNA: 3'- gCUACGCGGCaccGC-CgGCAGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 55132 | 0.7 | 0.250664 |
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Target: 5'- --cUGCGCCGUcGCGgcagcccaGCCGUCCG-CCu -3' miRNA: 3'- gcuACGCGGCAcCGC--------CGGCAGGCuGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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