Results 21 - 40 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12354 | 5' | -57 | NC_003324.1 | + | 1457 | 0.71 | 0.350944 |
Target: 5'- -cGCGACGuauccGGCAGCGgauuuCCGCCAUCUUc -3' miRNA: 3'- gaUGCUGU-----UCGUCGC-----GGCGGUGGAGu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 32297 | 0.7 | 0.359357 |
Target: 5'- -gGCGACAAcggccucugcGCGGCGCCG-CAUCUCc -3' miRNA: 3'- gaUGCUGUU----------CGUCGCGGCgGUGGAGu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 43150 | 0.7 | 0.375724 |
Target: 5'- -gACGGCGAGCuuguugagcgAGCGCCaauccgaGCCAgCCUCGa -3' miRNA: 3'- gaUGCUGUUCG----------UCGCGG-------CGGU-GGAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 36136 | 0.7 | 0.376599 |
Target: 5'- aUACGGCGuuGGUAGUGCgGCCAagguUCUCAu -3' miRNA: 3'- gAUGCUGU--UCGUCGCGgCGGU----GGAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 47842 | 0.7 | 0.385428 |
Target: 5'- uUAUGA--GGCGGCGCUGCCGCUg-- -3' miRNA: 3'- gAUGCUguUCGUCGCGGCGGUGGagu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 41554 | 0.69 | 0.422077 |
Target: 5'- -aACGAC-AGCGGCGCgGgCCuguuCCUCGc -3' miRNA: 3'- gaUGCUGuUCGUCGCGgC-GGu---GGAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 40640 | 0.66 | 0.597068 |
Target: 5'- -gACGACAcGGUAGUGCCGaucgaaaaCCACCa-- -3' miRNA: 3'- gaUGCUGU-UCGUCGCGGC--------GGUGGagu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 44918 | 0.66 | 0.590539 |
Target: 5'- -cACGGCA-GCAGCgggcacgccaaugacGCCGCCcCCUUc -3' miRNA: 3'- gaUGCUGUuCGUCG---------------CGGCGGuGGAGu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 53055 | 0.66 | 0.586194 |
Target: 5'- -gGCGGCAGGUcGCGCucgucucggcgCGUCACCUUu -3' miRNA: 3'- gaUGCUGUUCGuCGCG-----------GCGGUGGAGu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 31842 | 0.67 | 0.575357 |
Target: 5'- --cCGGCAGGCguuGGCGCCcCCAgUUCAa -3' miRNA: 3'- gauGCUGUUCG---UCGCGGcGGUgGAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 3925 | 0.67 | 0.564567 |
Target: 5'- -cAUGACGuGCGGCucaacgaaggaGCC-CCACCUCAu -3' miRNA: 3'- gaUGCUGUuCGUCG-----------CGGcGGUGGAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 24180 | 0.67 | 0.564567 |
Target: 5'- aCUACGACAAGgguauCAGCGCCaccguggcgcaGCgCACCg-- -3' miRNA: 3'- -GAUGCUGUUC-----GUCGCGG-----------CG-GUGGagu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 15909 | 0.66 | 0.597068 |
Target: 5'- -gGCGAUAgucuucGGCAGCuCgGCCGCCUgAa -3' miRNA: 3'- gaUGCUGU------UCGUCGcGgCGGUGGAgU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 15223 | 0.66 | 0.607971 |
Target: 5'- -gGCGGCGAGCcuuuucacguGGCGCCGUgucgaCGCCg-- -3' miRNA: 3'- gaUGCUGUUCG----------UCGCGGCG-----GUGGagu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 7361 | 0.66 | 0.607971 |
Target: 5'- uCUGCGugAuguAGCgAGCGaCCGUgAUCUCGg -3' miRNA: 3'- -GAUGCugU---UCG-UCGC-GGCGgUGGAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 16992 | 0.66 | 0.607971 |
Target: 5'- --gUGACGcuGGCA--GCCGCCACCUUg -3' miRNA: 3'- gauGCUGU--UCGUcgCGGCGGUGGAGu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 30606 | 0.66 | 0.618896 |
Target: 5'- -aGCGGCAgcGGCAGCgaagGUCGCCACg--- -3' miRNA: 3'- gaUGCUGU--UCGUCG----CGGCGGUGgagu -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 44673 | 0.66 | 0.629832 |
Target: 5'- uCUGCaACGGGCAGUuccucGCCGCCGugUCGc -3' miRNA: 3'- -GAUGcUGUUCGUCG-----CGGCGGUggAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 29035 | 0.66 | 0.629832 |
Target: 5'- -aACGAgcgcuugcuCAAGCAGCGCCaggcGgCACgCUCAa -3' miRNA: 3'- gaUGCU---------GUUCGUCGCGG----CgGUG-GAGU- -5' |
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12354 | 5' | -57 | NC_003324.1 | + | 25073 | 1.08 | 0.000723 |
Target: 5'- uCUACGACAAGCAGCGCCGCCACCUCAa -3' miRNA: 3'- -GAUGCUGUUCGUCGCGGCGGUGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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