Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12358 | 5' | -52.9 | NC_003324.1 | + | 44010 | 0.68 | 0.67735 |
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Target: 5'- --gGCUGCCGCCGggaUCAUgccgAGCgCUGCGa -3' miRNA: 3'- augUGGUGGUGGU---AGUAa---UCG-GACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 19634 | 0.68 | 0.666078 |
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Target: 5'- aACAUgACCGCCGUCGgcgAGUauaUGCGc -3' miRNA: 3'- aUGUGgUGGUGGUAGUaa-UCGg--ACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 33270 | 0.68 | 0.666078 |
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Target: 5'- gACGCCAUCGCCAuagUCAUcgacaUGGCCgacgaagGCa -3' miRNA: 3'- aUGUGGUGGUGGU---AGUA-----AUCGGa------CGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 30244 | 0.69 | 0.643444 |
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Target: 5'- gACGCCACCGCCGacacCAUcGGCgaugaUGCGg -3' miRNA: 3'- aUGUGGUGGUGGUa---GUAaUCGg----ACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 12317 | 0.7 | 0.575616 |
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Target: 5'- aACGCCGCCAUCAUacCAUUcGGCaucuCUGCGa -3' miRNA: 3'- aUGUGGUGGUGGUA--GUAA-UCG----GACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 1632 | 0.7 | 0.575616 |
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Target: 5'- aACGCCAUCGcaucCCGUCAcgaaacucGGCCUGCGc -3' miRNA: 3'- aUGUGGUGGU----GGUAGUaa------UCGGACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 37770 | 0.71 | 0.520342 |
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Target: 5'- aGCGCCuaugacaaACgCACCGUCAUgcGGCUUGCGa -3' miRNA: 3'- aUGUGG--------UG-GUGGUAGUAa-UCGGACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 10504 | 0.74 | 0.361498 |
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Target: 5'- gGCACaUACUAUCGUCA-UGGCCUGCa -3' miRNA: 3'- aUGUG-GUGGUGGUAGUaAUCGGACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 27115 | 1.07 | 0.002164 |
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Target: 5'- uUACACCACCACCAUCAUUAGCCUGCGc -3' miRNA: 3'- -AUGUGGUGGUGGUAGUAAUCGGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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