Results 21 - 40 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12364 | 5' | -62.3 | NC_003324.1 | + | 18855 | 0.69 | 0.252695 |
Target: 5'- -gUUGCCGAgaUCGAGgagcugcuCGUGGGCuCCGGCg -3' miRNA: 3'- uaGGCGGCU--AGCUC--------GCGCUCG-GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 54132 | 0.69 | 0.252695 |
Target: 5'- uUCCGCCGcagCGAaagccucuGCGCGccaaucGUCCGGCa -3' miRNA: 3'- uAGGCGGCua-GCU--------CGCGCu-----CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 17430 | 0.69 | 0.252695 |
Target: 5'- -cUCGUCGGUCauGAcGCG-GAGCCCGGUg -3' miRNA: 3'- uaGGCGGCUAG--CU-CGCgCUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 14812 | 0.69 | 0.258958 |
Target: 5'- -aUUGCUGAUUGAGUGuCGAGCCCa-- -3' miRNA: 3'- uaGGCGGCUAGCUCGC-GCUCGGGccg -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 10722 | 0.68 | 0.265346 |
Target: 5'- gGUCuCGCCaGUCGuAGCGacacgGAGCCaCGGCc -3' miRNA: 3'- -UAG-GCGGcUAGC-UCGCg----CUCGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 1591 | 0.68 | 0.271862 |
Target: 5'- -gCCGCCgGAUCcu-CGCuGGCCCGGCc -3' miRNA: 3'- uaGGCGG-CUAGcucGCGcUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 8192 | 0.68 | 0.285278 |
Target: 5'- -aCUGCCcggCG-GCGCGcGCCUGGCa -3' miRNA: 3'- uaGGCGGcuaGCuCGCGCuCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 52486 | 0.68 | 0.291484 |
Target: 5'- aGUUCGCCGAUCG-GCuCGAccucguagacuuuGCCgCGGCc -3' miRNA: 3'- -UAGGCGGCUAGCuCGcGCU-------------CGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 24276 | 0.67 | 0.313666 |
Target: 5'- uUCuCGUCGAUCuGGGCacgGCgGAGCuuGGCg -3' miRNA: 3'- uAG-GCGGCUAG-CUCG---CG-CUCGggCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 19926 | 0.67 | 0.328643 |
Target: 5'- aAUUCGCCGGUCuGAuaGaCGAGCCCaGUc -3' miRNA: 3'- -UAGGCGGCUAG-CUcgC-GCUCGGGcCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 40330 | 0.67 | 0.328643 |
Target: 5'- -aCCGCaCGcaaggcggcaGUCGAGCGgGAGCCa-GCa -3' miRNA: 3'- uaGGCG-GC----------UAGCUCGCgCUCGGgcCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 53654 | 0.67 | 0.336327 |
Target: 5'- -aCCGCCGugcggcaccuUgGAGCGcCGAGCUugauaCGGCg -3' miRNA: 3'- uaGGCGGCu---------AgCUCGC-GCUCGG-----GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 16522 | 0.67 | 0.344142 |
Target: 5'- ---aGCgGAUCGGccuGUGCGuGCUCGGCg -3' miRNA: 3'- uaggCGgCUAGCU---CGCGCuCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 18272 | 0.67 | 0.344142 |
Target: 5'- -aCCGCUGAcggaaUCGGccgacGCGCGGGCaaucgcugccCCGGCc -3' miRNA: 3'- uaGGCGGCU-----AGCU-----CGCGCUCG----------GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 21401 | 0.67 | 0.344142 |
Target: 5'- cGUCCGCgcAUCGAGCGUGuuCuuGGUg -3' miRNA: 3'- -UAGGCGgcUAGCUCGCGCucGggCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 26133 | 0.66 | 0.352086 |
Target: 5'- ---aGCCGcgcUUGAGCuaGAGCgCCGGCa -3' miRNA: 3'- uaggCGGCu--AGCUCGcgCUCG-GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 29666 | 0.66 | 0.352086 |
Target: 5'- uUCUGcCCGAggaaUCGAGCGaCGAGgCCUugaGGCc -3' miRNA: 3'- uAGGC-GGCU----AGCUCGC-GCUC-GGG---CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 43687 | 0.66 | 0.360159 |
Target: 5'- uGUCCGagCaGUCGAGCGCGAccGCCaaguaucaGGCc -3' miRNA: 3'- -UAGGCg-GcUAGCUCGCGCU--CGGg-------CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 48184 | 0.66 | 0.368361 |
Target: 5'- aGUCCGCU--UCGAGCaGC-AGCCacguCGGCg -3' miRNA: 3'- -UAGGCGGcuAGCUCG-CGcUCGG----GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 45974 | 0.66 | 0.368361 |
Target: 5'- --gCGCCGaAUCGAGgaGCGuGCCCGa- -3' miRNA: 3'- uagGCGGC-UAGCUCg-CGCuCGGGCcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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