Results 21 - 40 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12364 | 5' | -62.3 | NC_003324.1 | + | 10722 | 0.68 | 0.265346 |
Target: 5'- gGUCuCGCCaGUCGuAGCGacacgGAGCCaCGGCc -3' miRNA: 3'- -UAG-GCGGcUAGC-UCGCg----CUCGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 1591 | 0.68 | 0.271862 |
Target: 5'- -gCCGCCgGAUCcu-CGCuGGCCCGGCc -3' miRNA: 3'- uaGGCGG-CUAGcucGCGcUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 52486 | 0.68 | 0.291484 |
Target: 5'- aGUUCGCCGAUCG-GCuCGAccucguagacuuuGCCgCGGCc -3' miRNA: 3'- -UAGGCGGCUAGCuCGcGCU-------------CGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 8192 | 0.68 | 0.285278 |
Target: 5'- -aCUGCCcggCG-GCGCGcGCCUGGCa -3' miRNA: 3'- uaGGCGGcuaGCuCGCGCuCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 14812 | 0.69 | 0.258958 |
Target: 5'- -aUUGCUGAUUGAGUGuCGAGCCCa-- -3' miRNA: 3'- uaGGCGGCUAGCUCGC-GCUCGGGccg -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 18855 | 0.69 | 0.252695 |
Target: 5'- -gUUGCCGAgaUCGAGgagcugcuCGUGGGCuCCGGCg -3' miRNA: 3'- uaGGCGGCU--AGCUC--------GCGCUCG-GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 54132 | 0.69 | 0.252695 |
Target: 5'- uUCCGCCGcagCGAaagccucuGCGCGccaaucGUCCGGCa -3' miRNA: 3'- uAGGCGGCua-GCU--------CGCGCu-----CGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 20524 | 0.69 | 0.223236 |
Target: 5'- -cCCGCCGAcCGcaAGCGCccaucAGCgCCGGCa -3' miRNA: 3'- uaGGCGGCUaGC--UCGCGc----UCG-GGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 12100 | 0.69 | 0.252695 |
Target: 5'- gGUCUGUaccUGAUCgcgGAGCGCGcGGCCuCGGCc -3' miRNA: 3'- -UAGGCG---GCUAG---CUCGCGC-UCGG-GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 17430 | 0.69 | 0.252695 |
Target: 5'- -cUCGUCGGUCauGAcGCG-GAGCCCGGUg -3' miRNA: 3'- uaGGCGGCUAG--CU-CGCgCUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 40046 | 0.69 | 0.240544 |
Target: 5'- cGUCgGCCGuccacCGuaguuGCGCGGGUCUGGCc -3' miRNA: 3'- -UAGgCGGCua---GCu----CGCGCUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 2836 | 0.69 | 0.228885 |
Target: 5'- uUUCGCCGggCGAGgGgGAGCgucaaCGGCc -3' miRNA: 3'- uAGGCGGCuaGCUCgCgCUCGg----GCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 49395 | 0.69 | 0.223236 |
Target: 5'- -cCCGggcaaauacUCGAUCGcGaucgGCGAGCCCGGCa -3' miRNA: 3'- uaGGC---------GGCUAGCuCg---CGCUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 20551 | 0.7 | 0.201305 |
Target: 5'- cUCCGCgaugcggCGAUCGAGCGCGuucucaaugacGGCCgGaGCu -3' miRNA: 3'- uAGGCG-------GCUAGCUCGCGC-----------UCGGgC-CG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 54743 | 0.7 | 0.205432 |
Target: 5'- cAUCUGCCcGUUGGGUGCuucgaggaaggauuGAGCCgGGCg -3' miRNA: 3'- -UAGGCGGcUAGCUCGCG--------------CUCGGgCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 5965 | 0.7 | 0.21176 |
Target: 5'- -cCCGCCGAaUGgcuaugcAGCGCGAGCaCGGUg -3' miRNA: 3'- uaGGCGGCUaGC-------UCGCGCUCGgGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 4554 | 0.71 | 0.177574 |
Target: 5'- -gCCGCgccgUGAUCGAGCGCGcgcaGGUCCaGGCc -3' miRNA: 3'- uaGGCG----GCUAGCUCGCGC----UCGGG-CCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 54797 | 0.71 | 0.177574 |
Target: 5'- gAUgCGCCGAUCGAGCGUucGaCCGGa -3' miRNA: 3'- -UAgGCGGCUAGCUCGCGcuCgGGCCg -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 44576 | 0.71 | 0.164301 |
Target: 5'- -gCCGuCCGAUCGGGaaaugaGGGUCCGGCg -3' miRNA: 3'- uaGGC-GGCUAGCUCgcg---CUCGGGCCG- -5' |
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12364 | 5' | -62.3 | NC_003324.1 | + | 52667 | 0.72 | 0.159662 |
Target: 5'- gAUCCGCCGcau--GCGCGAGCguggauggucgauCCGGCg -3' miRNA: 3'- -UAGGCGGCuagcuCGCGCUCG-------------GGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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