Results 1 - 20 of 76 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12372 | 5' | -52.5 | NC_003324.1 | + | 48059 | 0.66 | 0.847917 |
Target: 5'- aUCugCuCUCGgcgaugaucgaUCGCUucaugAGGAUCGCGCCg -3' miRNA: 3'- gAGugG-GAGC-----------GGUGA-----UUUUAGCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 19263 | 0.66 | 0.847917 |
Target: 5'- aUCGCCCagCGCCGCgcagAGGAcCGCuaCa -3' miRNA: 3'- gAGUGGGa-GCGGUGa---UUUUaGCGcgG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 25403 | 0.66 | 0.856489 |
Target: 5'- cCUCugCCgaaUCGuCCACcAGAAaCGCGCa -3' miRNA: 3'- -GAGugGG---AGC-GGUGaUUUUaGCGCGg -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 52199 | 0.66 | 0.863172 |
Target: 5'- gCUCGCCUUCGCCGaccucgucaaaaUAAucGUCgGCGUCc -3' miRNA: 3'- -GAGUGGGAGCGGUg-----------AUUu-UAG-CGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 43675 | 0.66 | 0.880723 |
Target: 5'- aUCGgggaaCUCGCCAUgcgcugCGCGCCg -3' miRNA: 3'- gAGUgg---GAGCGGUGauuuuaGCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 37018 | 0.66 | 0.880723 |
Target: 5'- uUCGCCCUCGUCA------UCGC-CCu -3' miRNA: 3'- gAGUGGGAGCGGUgauuuuAGCGcGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 56957 | 0.66 | 0.875274 |
Target: 5'- -gCGCCCUCGCCGCcgcuccccaGCCc -3' miRNA: 3'- gaGUGGGAGCGGUGauuuuagcgCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 5565 | 0.66 | 0.872899 |
Target: 5'- --aACCUUCGCCGCgggcgucAUCG-GCCu -3' miRNA: 3'- gagUGGGAGCGGUGauuu---UAGCgCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 23660 | 0.66 | 0.864818 |
Target: 5'- cCUCGauaugCUUCGCCGgUAuccacggCGCGCCg -3' miRNA: 3'- -GAGUg----GGAGCGGUgAUuuua---GCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 17424 | 0.66 | 0.864818 |
Target: 5'- gUCGCCCUCGUCGgUcauGAcgCGgaGCCc -3' miRNA: 3'- gAGUGGGAGCGGUgAu--UUuaGCg-CGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 7638 | 0.66 | 0.856489 |
Target: 5'- -gUAUCUUCGC----GGAGUCGCGCCg -3' miRNA: 3'- gaGUGGGAGCGgugaUUUUAGCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 49439 | 0.66 | 0.880723 |
Target: 5'- gUCGCCCgaucgUCGacaCCGCUGu--UgGCGCCg -3' miRNA: 3'- gAGUGGG-----AGC---GGUGAUuuuAgCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 4129 | 0.66 | 0.880723 |
Target: 5'- gCUCuuCCUCGUgAUgucgaugAAGAgCGCGCCg -3' miRNA: 3'- -GAGugGGAGCGgUGa------UUUUaGCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 42706 | 0.66 | 0.872899 |
Target: 5'- -gCGCCCggCGCCGCgcgaugacuaUAAGcagacgaaguAUCGCGUCa -3' miRNA: 3'- gaGUGGGa-GCGGUG----------AUUU----------UAGCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 54621 | 0.66 | 0.862345 |
Target: 5'- aCUCGCCCcaUUGCUugaacaggaacggaACgccGGUCGCGCUg -3' miRNA: 3'- -GAGUGGG--AGCGG--------------UGauuUUAGCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 31344 | 0.66 | 0.847917 |
Target: 5'- --aGCCgUCGCCGCcgcAAAAaCGCGCa -3' miRNA: 3'- gagUGGgAGCGGUGa--UUUUaGCGCGg -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 49918 | 0.67 | 0.820844 |
Target: 5'- gCUCGCCUUCGCUGCccccAUCGCa-- -3' miRNA: 3'- -GAGUGGGAGCGGUGauuuUAGCGcgg -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 20992 | 0.67 | 0.801759 |
Target: 5'- gCUCACgUcggaUCGCCGCaauGAUggcCGCGCCg -3' miRNA: 3'- -GAGUGgG----AGCGGUGauuUUA---GCGCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 32084 | 0.67 | 0.799809 |
Target: 5'- uUCGgCCUCGCUGCgccucggcUCGuCGCCg -3' miRNA: 3'- gAGUgGGAGCGGUGauuuu---AGC-GCGG- -5' |
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12372 | 5' | -52.5 | NC_003324.1 | + | 17344 | 0.67 | 0.798831 |
Target: 5'- -aCGCCCUCGUCGuuauaacgauaggcCUugagGAGGUCGCGUa -3' miRNA: 3'- gaGUGGGAGCGGU--------------GA----UUUUAGCGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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