Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12376 | 3' | -57.9 | NC_003324.1 | + | 29970 | 0.66 | 0.601675 |
Target: 5'- uAGCGGCGGCagccggguccgcaucGAUCccGGCCGgUGCacucugGCg -3' miRNA: 3'- -UCGCUGCUG---------------CUAGu-CCGGCgACGa-----CG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 18238 | 0.66 | 0.597369 |
Target: 5'- cGGCGGucagguugccauCGACGA-CAGaGCCGCgaccGCUGa -3' miRNA: 3'- -UCGCU------------GCUGCUaGUC-CGGCGa---CGACg -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 30062 | 0.66 | 0.596293 |
Target: 5'- -aCGcCGcCGGUCGcgacaccGGCCGCUGCgGCu -3' miRNA: 3'- ucGCuGCuGCUAGU-------CCGGCGACGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 5350 | 0.66 | 0.586623 |
Target: 5'- uAGCGAgauCGAgGAuaUCGccuGCCGCUGgUGCg -3' miRNA: 3'- -UCGCU---GCUgCU--AGUc--CGGCGACgACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 1232 | 0.66 | 0.554637 |
Target: 5'- aAGCGAuCGGCaaacagCAGGCCGCUGa--- -3' miRNA: 3'- -UCGCU-GCUGcua---GUCCGGCGACgacg -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 12540 | 0.66 | 0.554637 |
Target: 5'- aGGCGACGGuuCGAUCAGGCUGgUuacggaacaGCUu- -3' miRNA: 3'- -UCGCUGCU--GCUAGUCCGGCgA---------CGAcg -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 35795 | 0.66 | 0.553579 |
Target: 5'- uGGCGAUGGCGAUCuGGggguugaCCGgaGCcaGCu -3' miRNA: 3'- -UCGCUGCUGCUAGuCC-------GGCgaCGa-CG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 27060 | 0.67 | 0.544084 |
Target: 5'- cGCGACGcgGCGuuaccguUguGGCCGgUGCgGCu -3' miRNA: 3'- uCGCUGC--UGCu------AguCCGGCgACGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 8407 | 0.67 | 0.544084 |
Target: 5'- cGUGAUG-CgGGUCAGGgauuuCCGCcgGCUGCa -3' miRNA: 3'- uCGCUGCuG-CUAGUCC-----GGCGa-CGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 28541 | 0.67 | 0.533597 |
Target: 5'- cGUGACGugGAacaAGGCCgGCaacgGCUGg -3' miRNA: 3'- uCGCUGCugCUag-UCCGG-CGa---CGACg -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 17136 | 0.67 | 0.533597 |
Target: 5'- cGGUGugG-CGAUCacgaAGGagaCGCUGCaGCg -3' miRNA: 3'- -UCGCugCuGCUAG----UCCg--GCGACGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 29738 | 0.67 | 0.51182 |
Target: 5'- --aGACGGCGGacucgaucccggcUCAGGCgGCggcGUUGCg -3' miRNA: 3'- ucgCUGCUGCU-------------AGUCCGgCGa--CGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 38436 | 0.67 | 0.502599 |
Target: 5'- -aCGACGGCGAcCAGcGCCGCcccGgUGCc -3' miRNA: 3'- ucGCUGCUGCUaGUC-CGGCGa--CgACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 42742 | 0.67 | 0.502599 |
Target: 5'- uGGUGGCccGGCGAau-GGCCuuGCUGUUGCa -3' miRNA: 3'- -UCGCUG--CUGCUaguCCGG--CGACGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 35464 | 0.67 | 0.492439 |
Target: 5'- cGCgGGCGGcCGAgCAGGCUGCaGCaGCa -3' miRNA: 3'- uCG-CUGCU-GCUaGUCCGGCGaCGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 9002 | 0.68 | 0.486389 |
Target: 5'- uGuCGACGAgGGUCAGcggaaaccaaucacuGCCGCccGCUGUg -3' miRNA: 3'- uC-GCUGCUgCUAGUC---------------CGGCGa-CGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 16420 | 0.68 | 0.472412 |
Target: 5'- cGCGAuCGACGGcaacgccgUCGcGGaCCGCUGCgucUGCu -3' miRNA: 3'- uCGCU-GCUGCU--------AGU-CC-GGCGACG---ACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 14592 | 0.68 | 0.452804 |
Target: 5'- aAGCGGCcGGCGGcgCAGGCgGUaGCgugGCg -3' miRNA: 3'- -UCGCUG-CUGCUa-GUCCGgCGaCGa--CG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 35030 | 0.68 | 0.442211 |
Target: 5'- cAGCGGCGGCGG-CAGcagcggccugaucGUCGUcGCUGCc -3' miRNA: 3'- -UCGCUGCUGCUaGUC-------------CGGCGaCGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 13512 | 0.68 | 0.441255 |
Target: 5'- cGCGGCGGCGGugcacuggacaccUCAgucgccuguaucuGGCCGa-GCUGCa -3' miRNA: 3'- uCGCUGCUGCU-------------AGU-------------CCGGCgaCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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