Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12376 | 3' | -57.9 | NC_003324.1 | + | 52658 | 0.7 | 0.362023 |
Target: 5'- gAGCGuCGGCGAUguugacggugaUAGGCUGUcucaugcuugUGCUGCc -3' miRNA: 3'- -UCGCuGCUGCUA-----------GUCCGGCG----------ACGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 5350 | 0.66 | 0.586623 |
Target: 5'- uAGCGAgauCGAgGAuaUCGccuGCCGCUGgUGCg -3' miRNA: 3'- -UCGCU---GCUgCU--AGUc--CGGCGACgACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 17136 | 0.67 | 0.533597 |
Target: 5'- cGGUGugG-CGAUCacgaAGGagaCGCUGCaGCg -3' miRNA: 3'- -UCGCugCuGCUAG----UCCg--GCGACGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 13512 | 0.68 | 0.441255 |
Target: 5'- cGCGGCGGCGGugcacuggacaccUCAgucgccuguaucuGGCCGa-GCUGCa -3' miRNA: 3'- uCGCUGCUGCU-------------AGU-------------CCGGCgaCGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 32382 | 0.68 | 0.433648 |
Target: 5'- uGCGGCGcCGcgCagAGGCCGUUGUcGCc -3' miRNA: 3'- uCGCUGCuGCuaG--UCCGGCGACGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 28541 | 0.67 | 0.533597 |
Target: 5'- cGUGACGugGAacaAGGCCgGCaacgGCUGg -3' miRNA: 3'- uCGCUGCugCUag-UCCGG-CGa---CGACg -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 35464 | 0.67 | 0.492439 |
Target: 5'- cGCgGGCGGcCGAgCAGGCUGCaGCaGCa -3' miRNA: 3'- uCG-CUGCU-GCUaGUCCGGCGaCGaCG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 16420 | 0.68 | 0.472412 |
Target: 5'- cGCGAuCGACGGcaacgccgUCGcGGaCCGCUGCgucUGCu -3' miRNA: 3'- uCGCU-GCUGCU--------AGU-CC-GGCGACG---ACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 39668 | 0.7 | 0.370515 |
Target: 5'- uGuCGAgGACGAUCcggAGGCUGgUGCUGa -3' miRNA: 3'- uC-GCUgCUGCUAG---UCCGGCgACGACg -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 13164 | 0.73 | 0.215346 |
Target: 5'- cGuCGGCGGCGGUCGgcGGCgGCgguaccgGCUGCa -3' miRNA: 3'- uC-GCUGCUGCUAGU--CCGgCGa------CGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 35124 | 1.1 | 0.000464 |
Target: 5'- cAGCGACGACGAUCAGGCCGCUGCUGCc -3' miRNA: 3'- -UCGCUGCUGCUAGUCCGGCGACGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 38436 | 0.67 | 0.502599 |
Target: 5'- -aCGACGGCGAcCAGcGCCGCcccGgUGCc -3' miRNA: 3'- ucGCUGCUGCUaGUC-CGGCGa--CgACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 29738 | 0.67 | 0.51182 |
Target: 5'- --aGACGGCGGacucgaucccggcUCAGGCgGCggcGUUGCg -3' miRNA: 3'- ucgCUGCUGCU-------------AGUCCGgCGa--CGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 40936 | 0.69 | 0.379143 |
Target: 5'- cGCGGgGGCaGAUCAGcaaucaCCGCUGCgagGCg -3' miRNA: 3'- uCGCUgCUG-CUAGUCc-----GGCGACGa--CG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 29650 | 0.7 | 0.370515 |
Target: 5'- gAGCGACGAgGccuUgAGGCCGCUugaGCcccgUGCa -3' miRNA: 3'- -UCGCUGCUgCu--AgUCCGGCGA---CG----ACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 42742 | 0.67 | 0.502599 |
Target: 5'- uGGUGGCccGGCGAau-GGCCuuGCUGUUGCa -3' miRNA: 3'- -UCGCUG--CUGCUaguCCGG--CGACGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 26319 | 0.73 | 0.232685 |
Target: 5'- cGUGGCaGAUGGUCAGGgagaccuacUUGCUGCUGCc -3' miRNA: 3'- uCGCUG-CUGCUAGUCC---------GGCGACGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 32911 | 0.8 | 0.080766 |
Target: 5'- cAGUGGCGAUGAUCgAGGCgGCUGCccgccaUGCg -3' miRNA: 3'- -UCGCUGCUGCUAG-UCCGgCGACG------ACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 9002 | 0.68 | 0.486389 |
Target: 5'- uGuCGACGAgGGUCAGcggaaaccaaucacuGCCGCccGCUGUg -3' miRNA: 3'- uC-GCUGCUgCUAGUC---------------CGGCGa-CGACG- -5' |
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12376 | 3' | -57.9 | NC_003324.1 | + | 10681 | 0.69 | 0.379143 |
Target: 5'- gGGCGACuggggGACGGUU--GCCGUUGcCUGCa -3' miRNA: 3'- -UCGCUG-----CUGCUAGucCGGCGAC-GACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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