Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12400 | 3' | -61.3 | NC_003324.1 | + | 41611 | 0.69 | 0.262453 |
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Target: 5'- cCUCUGCGAGa---GCCACGCCCAcCa -3' miRNA: 3'- -GGGACGCUCgacgUGGUGCGGGUcGc -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 41642 | 0.67 | 0.37395 |
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Target: 5'- aCCUGCGAGgaacagGC-CCGCGCCgcugucguUAGCGu -3' miRNA: 3'- gGGACGCUCga----CGuGGUGCGG--------GUCGC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 42725 | 0.76 | 0.084749 |
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Target: 5'- gCCUUGCu-GUUGCAgauCCGCGCCCGGCGc -3' miRNA: 3'- -GGGACGcuCGACGU---GGUGCGGGUCGC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 42880 | 0.67 | 0.349275 |
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Target: 5'- gCCC-GCGAGgauCUGCgACCgagaACGaCCCAGCGc -3' miRNA: 3'- -GGGaCGCUC---GACG-UGG----UGC-GGGUCGC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 43196 | 0.72 | 0.161727 |
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Target: 5'- aCCUGCGGGacuuugGCAUUAUGCCCgcuGGCGg -3' miRNA: 3'- gGGACGCUCga----CGUGGUGCGGG---UCGC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 45770 | 0.68 | 0.318217 |
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Target: 5'- gCCCUGCucacgccucgccGAGCcccUGCAUCuauUGCCCAGgGg -3' miRNA: 3'- -GGGACG------------CUCG---ACGUGGu--GCGGGUCgC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 49334 | 0.68 | 0.333482 |
|
Target: 5'- aUCUGCacguGCUGUGCgGCGCCaaCAGCGg -3' miRNA: 3'- gGGACGcu--CGACGUGgUGCGG--GUCGC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 50516 | 1.09 | 0.000284 |
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Target: 5'- gCCCUGCGAGCUGCACCACGCCCAGCGc -3' miRNA: 3'- -GGGACGCUCGACGUGGUGCGGGUCGC- -5' |
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| 12400 | 3' | -61.3 | NC_003324.1 | + | 53804 | 0.67 | 0.35737 |
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Target: 5'- uUCUGgugGGGCUGCACCAaggucuCGCCgGGCu -3' miRNA: 3'- gGGACg--CUCGACGUGGU------GCGGgUCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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