Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12413 | 5' | -54.8 | NC_003324.1 | + | 14470 | 0.66 | 0.729639 |
Target: 5'- uGCGCGCucgguuGCG-GCGCguuGAUUGGCAGc -3' miRNA: 3'- -CGCGUGu-----UGUaCGCGgu-CUAGCCGUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 30734 | 0.66 | 0.729639 |
Target: 5'- cCGCGCAGCuaucuccgugGCGUCGGGgaaUCGGCc- -3' miRNA: 3'- cGCGUGUUGua--------CGCGGUCU---AGCCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 23191 | 0.66 | 0.729639 |
Target: 5'- aGCGCgguaccgcucuGCAuCGUGCGCCAGAUgaagcucuucUGGUg- -3' miRNA: 3'- -CGCG-----------UGUuGUACGCGGUCUA----------GCCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 37512 | 0.66 | 0.718816 |
Target: 5'- uGUGCGCGGCaAUGCGCUcGAUa-GCAAc -3' miRNA: 3'- -CGCGUGUUG-UACGCGGuCUAgcCGUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 11838 | 0.66 | 0.718816 |
Target: 5'- cUGCGCGACcgG-GCCGGGcaCGGCAu -3' miRNA: 3'- cGCGUGUUGuaCgCGGUCUa-GCCGUu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 54047 | 0.66 | 0.718816 |
Target: 5'- gGCGCGCAgaggcuuucGC-UGCGgCGGAaUCGGCc- -3' miRNA: 3'- -CGCGUGU---------UGuACGCgGUCU-AGCCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 53412 | 0.66 | 0.707903 |
Target: 5'- uGCGCACGGaugaagucCAUGCGCUcaa-CGGCGAc -3' miRNA: 3'- -CGCGUGUU--------GUACGCGGucuaGCCGUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 44313 | 0.66 | 0.707903 |
Target: 5'- cGUGCACAGCGuUGCGCgC-GAUCaGCu- -3' miRNA: 3'- -CGCGUGUUGU-ACGCG-GuCUAGcCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 1469 | 0.66 | 0.696914 |
Target: 5'- -aGCACcgUcgGCGCaucgaugucaCGGAUCGGCAu -3' miRNA: 3'- cgCGUGuuGuaCGCG----------GUCUAGCCGUu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 1602 | 0.66 | 0.696914 |
Target: 5'- cGCGCACcAC-UGcCGCCGGAUCcucgcuGGCc- -3' miRNA: 3'- -CGCGUGuUGuAC-GCGGUCUAG------CCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 39450 | 0.66 | 0.696914 |
Target: 5'- -aGUACAGguucucgAUGCGCCacaucGGGUCGGCGAa -3' miRNA: 3'- cgCGUGUUg------UACGCGG-----UCUAGCCGUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 43997 | 0.66 | 0.6792 |
Target: 5'- uGCGgGCucgguGCcgGCGCCaaaggcuucaacaagGGAUCGGCu- -3' miRNA: 3'- -CGCgUGu----UGuaCGCGG---------------UCUAGCCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 44593 | 0.66 | 0.674752 |
Target: 5'- aCGCACcGCAUGCgcguGCCGuccGAUCGGgAAa -3' miRNA: 3'- cGCGUGuUGUACG----CGGU---CUAGCCgUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 50127 | 0.66 | 0.674752 |
Target: 5'- gGCGC-CAugccuuuggaGCGUGCccGCCAGAUcguuaCGGCGAu -3' miRNA: 3'- -CGCGuGU----------UGUACG--CGGUCUA-----GCCGUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 40495 | 0.67 | 0.663602 |
Target: 5'- gGCG-ACGACGaaGCGCCAGAcgaaGGCGAa -3' miRNA: 3'- -CGCgUGUUGUa-CGCGGUCUag--CCGUU- -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 29754 | 0.67 | 0.663602 |
Target: 5'- uCGUACGccuCgAUGCGCCcaaGGAUCGGCu- -3' miRNA: 3'- cGCGUGUu--G-UACGCGG---UCUAGCCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 25888 | 0.67 | 0.663602 |
Target: 5'- gGCGCGCAgGCGUGCGUCucgacuauGA-CGGUAc -3' miRNA: 3'- -CGCGUGU-UGUACGCGGu-------CUaGCCGUu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 18104 | 0.67 | 0.663602 |
Target: 5'- uCGCAUGGCccgugGCGCCGGccaAUCGGUAu -3' miRNA: 3'- cGCGUGUUGua---CGCGGUC---UAGCCGUu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 17355 | 0.67 | 0.652422 |
Target: 5'- cGUGCAUGACGUGguCGCaCAGGcgcUCGGCu- -3' miRNA: 3'- -CGCGUGUUGUAC--GCG-GUCU---AGCCGuu -5' |
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12413 | 5' | -54.8 | NC_003324.1 | + | 21862 | 0.67 | 0.652422 |
Target: 5'- aGCGCucgACAACAUGuCGCCGGAcgaguuccUCGcccuGCAGa -3' miRNA: 3'- -CGCG---UGUUGUAC-GCGGUCU--------AGC----CGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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