Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12419 | 3' | -54.3 | NC_003324.1 | + | 45995 | 0.67 | 0.716305 |
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Target: 5'- -uGCUuc-GCUUCCGaagcGGCGGCGCCg -3' miRNA: 3'- guUGGcuuUGGAGGCaa--CCGCCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 49588 | 0.67 | 0.713099 |
|
Target: 5'- aCGGCCGAGagugccagaaugccGCCcUCGUUGcggacCGGCACCu -3' miRNA: 3'- -GUUGGCUU--------------UGGaGGCAACc----GCCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 49595 | 0.69 | 0.618235 |
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Target: 5'- aGGCUGAccaGACCUauggcgauaCCGUcauucUGGCaGGCGCCa -3' miRNA: 3'- gUUGGCU---UUGGA---------GGCA-----ACCG-CCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 53643 | 0.67 | 0.758212 |
|
Target: 5'- cCAAaaGAAcuACCgCCGU--GCGGCACCu -3' miRNA: 3'- -GUUggCUU--UGGaGGCAacCGCCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 54877 | 0.67 | 0.7479 |
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Target: 5'- --cCCGGAugCUCgCGaUGuCGGCGCCc -3' miRNA: 3'- guuGGCUUugGAG-GCaACcGCCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 56207 | 0.72 | 0.469498 |
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Target: 5'- gCGGCCGAuGugUUCCGgguUGGCGGCGa- -3' miRNA: 3'- -GUUGGCU-UugGAGGCa--ACCGCCGUgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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