Results 21 - 38 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12438 | 5' | -54.9 | NC_003324.1 | + | 31014 | 0.77 | 0.228748 |
Target: 5'- cUCgUCGAUCgcUUGGUAGCGAccgGCGCGGu -3' miRNA: 3'- -AGgAGCUAG--AGCCGUUGCUa--CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 15118 | 0.72 | 0.438282 |
Target: 5'- gCCaUCGGUCUCGGCGuCGAcacgGCGCc- -3' miRNA: 3'- aGG-AGCUAGAGCCGUuGCUa---CGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 31845 | 0.71 | 0.45766 |
Target: 5'- cUUCUUGGcauUCUCGGCGAUGAucUGCGCc- -3' miRNA: 3'- -AGGAGCU---AGAGCCGUUGCU--ACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 44287 | 0.71 | 0.45766 |
Target: 5'- gCCUCGAUCUgucucgacgaacCGGgcGCGGUgGCGCGGc -3' miRNA: 3'- aGGAGCUAGA------------GCCguUGCUA-CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 1516 | 0.7 | 0.549883 |
Target: 5'- gCCagCGAggaUCcggCGGCAGUGGUGCGCGGc -3' miRNA: 3'- aGGa-GCU---AGa--GCCGUUGCUACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 17555 | 0.7 | 0.549883 |
Target: 5'- aCCaaaGGUC-CGGCAugcCGAUGCGCGa -3' miRNA: 3'- aGGag-CUAGaGCCGUu--GCUACGCGCc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 26482 | 0.7 | 0.560529 |
Target: 5'- -gCUCGAcCUCGGCAGCGcucgacauaccGUcgaauugcgugGCGCGGg -3' miRNA: 3'- agGAGCUaGAGCCGUUGC-----------UA-----------CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 44505 | 0.68 | 0.645957 |
Target: 5'- uUCC-CGAUCggaCGGCAcgcgcauGCGGUGCGUc- -3' miRNA: 3'- -AGGaGCUAGa--GCCGU-------UGCUACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 29207 | 0.68 | 0.657895 |
Target: 5'- cUUCUCG-UC-CGGCAgcacuACGGUGCGCu- -3' miRNA: 3'- -AGGAGCuAGaGCCGU-----UGCUACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 18782 | 1.12 | 0.000893 |
Target: 5'- cUCCUCGAUCUCGGCAACGAUGCGCGGg -3' miRNA: 3'- -AGGAGCUAGAGCCGUUGCUACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 54434 | 0.68 | 0.679518 |
Target: 5'- gCCUCGA-CUgggucauagcCGGCGGCGAga-GCGGa -3' miRNA: 3'- aGGAGCUaGA----------GCCGUUGCUacgCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 19150 | 0.67 | 0.702032 |
Target: 5'- gUCCUUGAUCUUcuggagcgucugcuuGUAGCGGuccucUGCGCGGc -3' miRNA: 3'- -AGGAGCUAGAGc--------------CGUUGCU-----ACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 24278 | 0.67 | 0.711598 |
Target: 5'- cUCgUCGAUCUgGGCAcggcggagcuugGCGAaggcGuCGCGGa -3' miRNA: 3'- -AGgAGCUAGAgCCGU------------UGCUa---C-GCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 39773 | 0.67 | 0.732621 |
Target: 5'- -gCUCGAugaUCUCGGC-ACGGUGCa--- -3' miRNA: 3'- agGAGCU---AGAGCCGuUGCUACGcgcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 21323 | 0.67 | 0.74299 |
Target: 5'- aUCCUCGAUUUCGcGCGuuuucaGAuUGCccuuGCGGa -3' miRNA: 3'- -AGGAGCUAGAGC-CGUug----CU-ACG----CGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 5278 | 0.66 | 0.763391 |
Target: 5'- aUCCUCGAUCUCG-CuAUGAUcccgGC-CGGa -3' miRNA: 3'- -AGGAGCUAGAGCcGuUGCUA----CGcGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 38003 | 0.66 | 0.763391 |
Target: 5'- gCCggagCGGUUgCGGCGcACGggGCGCGu -3' miRNA: 3'- aGGa---GCUAGaGCCGU-UGCuaCGCGCc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 12481 | 0.66 | 0.772405 |
Target: 5'- aUCgUCGAUCUCGGUcACGcgGUcugcuacGCGu -3' miRNA: 3'- -AGgAGCUAGAGCCGuUGCuaCG-------CGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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