Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12446 | 5' | -56.1 | NC_003324.1 | + | 26487 | 1.11 | 0.000716 |
Target: 5'- aCCUCGGCAGCGCUCGACAUACCGUCGa -3' miRNA: 3'- -GGAGCCGUCGCGAGCUGUAUGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 44101 | 0.79 | 0.137255 |
Target: 5'- aCCUC-GCAGCGCUCGGCAUGaucCCGgCGg -3' miRNA: 3'- -GGAGcCGUCGCGAGCUGUAU---GGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 22094 | 0.77 | 0.199432 |
Target: 5'- ---aGGCuGCGCUCGACAUGCgccuCGUCGa -3' miRNA: 3'- ggagCCGuCGCGAGCUGUAUG----GCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 25586 | 0.75 | 0.264312 |
Target: 5'- uCCUCGGCGGUGa-UGACAUAguCUGUCGg -3' miRNA: 3'- -GGAGCCGUCGCgaGCUGUAU--GGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 13439 | 0.73 | 0.322332 |
Target: 5'- uCCguaCGGCAGCGCgucgcauguuuggucCGGCGcggcUGCCGUCGa -3' miRNA: 3'- -GGa--GCCGUCGCGa--------------GCUGU----AUGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 43241 | 0.72 | 0.396446 |
Target: 5'- gCUCGGauuGGCGCUCGcucaACAagcucGCCGUCGa -3' miRNA: 3'- gGAGCCg--UCGCGAGC----UGUa----UGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 1991 | 0.72 | 0.36181 |
Target: 5'- --aCGGCuuCGCUCGACGUucucaACCGUCa -3' miRNA: 3'- ggaGCCGucGCGAGCUGUA-----UGGCAGc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 51784 | 0.71 | 0.433145 |
Target: 5'- gCCUCGcugauauccuGCAGgCGCUCGACGcgaaCGUCGg -3' miRNA: 3'- -GGAGC----------CGUC-GCGAGCUGUaug-GCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 39500 | 0.71 | 0.411799 |
Target: 5'- uCgUCGGCAGagaacccggccaaaUGCUCGuACAUcaGCCGUCGu -3' miRNA: 3'- -GgAGCCGUC--------------GCGAGC-UGUA--UGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 11009 | 0.71 | 0.442625 |
Target: 5'- aCCcCGGCGGUGUUgGcauACAUACCGaUCGg -3' miRNA: 3'- -GGaGCCGUCGCGAgC---UGUAUGGC-AGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 32403 | 0.7 | 0.481668 |
Target: 5'- aCCUCGGCcGCGCUCu---UACCG-CGc -3' miRNA: 3'- -GGAGCCGuCGCGAGcuguAUGGCaGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 38354 | 0.7 | 0.452221 |
Target: 5'- aCCggGGCGGCGCUgGuC--GCCGUCGu -3' miRNA: 3'- -GGagCCGUCGCGAgCuGuaUGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 25325 | 0.7 | 0.459005 |
Target: 5'- gCCUUGGCuuuaagggcggccaGGUGCUCGA---ACCGUCa -3' miRNA: 3'- -GGAGCCG--------------UCGCGAGCUguaUGGCAGc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 13165 | 0.7 | 0.47076 |
Target: 5'- gCgUCGGCGGCGgUCGGCggcggcgGUACCGg-- -3' miRNA: 3'- -GgAGCCGUCGCgAGCUG-------UAUGGCagc -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 47670 | 0.69 | 0.512014 |
Target: 5'- --cCGGU-GCGCUgGACAguuCCGUCGg -3' miRNA: 3'- ggaGCCGuCGCGAgCUGUau-GGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 18897 | 0.69 | 0.55363 |
Target: 5'- gCUCGGUcGCGUUCGGC-UGCgCGcUCGa -3' miRNA: 3'- gGAGCCGuCGCGAGCUGuAUG-GC-AGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 33755 | 0.69 | 0.512014 |
Target: 5'- gCCUUGGCGGCaGCgcaGGCGUAUCGagCGu -3' miRNA: 3'- -GGAGCCGUCG-CGag-CUGUAUGGCa-GC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 49724 | 0.69 | 0.54312 |
Target: 5'- -aUCGGCAGCGUcgUCGAaGUugCGaUCGu -3' miRNA: 3'- ggAGCCGUCGCG--AGCUgUAugGC-AGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 6377 | 0.68 | 0.596195 |
Target: 5'- ---aGGCuauuGUGCUCGcCAaGCCGUCGg -3' miRNA: 3'- ggagCCGu---CGCGAGCuGUaUGGCAGC- -5' |
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12446 | 5' | -56.1 | NC_003324.1 | + | 12360 | 0.68 | 0.596195 |
Target: 5'- gCUCGGCAGCauuCUCGugGUAgUGaCGa -3' miRNA: 3'- gGAGCCGUCGc--GAGCugUAUgGCaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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