Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12447 | 5' | -57 | NC_003324.1 | + | 35014 | 0.75 | 0.185109 |
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Target: 5'- gCUGCCGCaGCagCAGCAgcGGCGGCGg-- -3' miRNA: 3'- -GACGGCG-CGa-GUUGUa-CCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 36271 | 0.7 | 0.352135 |
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Target: 5'- aUGUCGCGCaacaucaguaugcugCAGCAggucGGCGGCGUg- -3' miRNA: 3'- gACGGCGCGa--------------GUUGUa---CCGCCGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 39989 | 0.68 | 0.452078 |
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Target: 5'- -cGUCGCGCcgAGCGUGGCgagGGUGUUGg -3' miRNA: 3'- gaCGGCGCGagUUGUACCG---CCGCAAU- -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 40599 | 0.79 | 0.094681 |
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Target: 5'- -cGCCGCGCUCGAuCGUGGCuccaucgGGCGUg- -3' miRNA: 3'- gaCGGCGCGAGUU-GUACCG-------CCGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 41486 | 0.66 | 0.588509 |
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Target: 5'- -aGCCacggGCGCUCGuCGUGGCGaGCa--- -3' miRNA: 3'- gaCGG----CGCGAGUuGUACCGC-CGcaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 44097 | 0.69 | 0.404265 |
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Target: 5'- -cGCaGCGCUCGGCAUgaucccGGCGGCa--- -3' miRNA: 3'- gaCGgCGCGAGUUGUA------CCGCCGcaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 47724 | 0.7 | 0.377153 |
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Target: 5'- gUGCCacaGCUCGACgAUGGCGGCc--- -3' miRNA: 3'- gACGGcg-CGAGUUG-UACCGCCGcaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 53986 | 0.71 | 0.311054 |
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Target: 5'- uUGCCGCGCUgAugAUcuuGCGGCGg-- -3' miRNA: 3'- gACGGCGCGAgUugUAc--CGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 54426 | 0.68 | 0.448139 |
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Target: 5'- gCUGCCGgGcCUCGACugggucauagccGGCGGCGa-- -3' miRNA: 3'- -GACGGCgC-GAGUUGua----------CCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 56674 | 0.67 | 0.554806 |
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Target: 5'- gUGCCGcCGUcaauucgUCAGCGauaGCGGCGUUAu -3' miRNA: 3'- gACGGC-GCG-------AGUUGUac-CGCCGCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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