Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12447 | 5' | -57 | NC_003324.1 | + | 13169 | 0.68 | 0.482204 |
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Target: 5'- -gGCUGCGUcggCGGCGgucGGCGGCGg-- -3' miRNA: 3'- gaCGGCGCGa--GUUGUa--CCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 4636 | 0.68 | 0.482204 |
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Target: 5'- cCUGCgCGCGCUCGAUcacgGcGCGGCu--- -3' miRNA: 3'- -GACG-GCGCGAGUUGua--C-CGCCGcaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 22093 | 0.68 | 0.482204 |
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Target: 5'- -gGCUGCGCUCGACAUGcGCcuCGUc- -3' miRNA: 3'- gaCGGCGCGAGUUGUAC-CGccGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 56674 | 0.67 | 0.554806 |
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Target: 5'- gUGCCGcCGUcaauucgUCAGCGauaGCGGCGUUAu -3' miRNA: 3'- gACGGC-GCG-------AGUUGUac-CGCCGCAAU- -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 29994 | 0.66 | 0.577587 |
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Target: 5'- -gGCCGCGCcgucCAGCAUGGCcagcaGCGc-- -3' miRNA: 3'- gaCGGCGCGa---GUUGUACCGc----CGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 30137 | 0.66 | 0.577587 |
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Target: 5'- gUGUCGCGaccggCGGCGUgaugGGCGGCGg-- -3' miRNA: 3'- gACGGCGCga---GUUGUA----CCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 24404 | 0.66 | 0.588509 |
|
Target: 5'- -gGaCCGCGUUgcCGGCAUGGUGcGCGUc- -3' miRNA: 3'- gaC-GGCGCGA--GUUGUACCGC-CGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 28917 | 0.66 | 0.599468 |
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Target: 5'- -aGCgCGUGCUCgAACA-GGCuGGCGUc- -3' miRNA: 3'- gaCG-GCGCGAG-UUGUaCCG-CCGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 9730 | 0.66 | 0.599468 |
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Target: 5'- -cGCCcaGCGCgUUGGCGUugccGGCGGUGUUGg -3' miRNA: 3'- gaCGG--CGCG-AGUUGUA----CCGCCGCAAU- -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 40599 | 0.79 | 0.094681 |
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Target: 5'- -cGCCGCGCUCGAuCGUGGCuccaucgGGCGUg- -3' miRNA: 3'- gaCGGCGCGAGUU-GUACCG-------CCGCAau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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