Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12447 | 5' | -57 | NC_003324.1 | + | 30137 | 0.66 | 0.577587 |
Target: 5'- gUGUCGCGaccggCGGCGUgaugGGCGGCGg-- -3' miRNA: 3'- gACGGCGCga---GUUGUA----CCGCCGCaau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 29994 | 0.66 | 0.577587 |
Target: 5'- -gGCCGCGCcgucCAGCAUGGCcagcaGCGc-- -3' miRNA: 3'- gaCGGCGCGa---GUUGUACCGc----CGCaau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 9649 | 0.66 | 0.577587 |
Target: 5'- -cGCCGgcaaCGC-CAACGcgcugGGCGGCGUg- -3' miRNA: 3'- gaCGGC----GCGaGUUGUa----CCGCCGCAau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 24404 | 0.66 | 0.588509 |
Target: 5'- -gGaCCGCGUUgcCGGCAUGGUGcGCGUc- -3' miRNA: 3'- gaC-GGCGCGA--GUUGUACCGC-CGCAau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 41486 | 0.66 | 0.588509 |
Target: 5'- -aGCCacggGCGCUCGuCGUGGCGaGCa--- -3' miRNA: 3'- gaCGG----CGCGAGUuGUACCGC-CGcaau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 21918 | 0.66 | 0.588509 |
Target: 5'- aUGCCGCGCUgGGCuucccacuucuuGUGGauGCGg-- -3' miRNA: 3'- gACGGCGCGAgUUG------------UACCgcCGCaau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 24022 | 0.66 | 0.588509 |
Target: 5'- gCUGCgUGCGCUUggagAAgAUGGCGGCc--- -3' miRNA: 3'- -GACG-GCGCGAG----UUgUACCGCCGcaau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 9730 | 0.66 | 0.599468 |
Target: 5'- -cGCCcaGCGCgUUGGCGUugccGGCGGUGUUGg -3' miRNA: 3'- gaCGG--CGCG-AGUUGUA----CCGCCGCAAU- -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 23539 | 0.66 | 0.599468 |
Target: 5'- gUGCC-C-CaCAGCGUGGCGGCGg-- -3' miRNA: 3'- gACGGcGcGaGUUGUACCGCCGCaau -5' |
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12447 | 5' | -57 | NC_003324.1 | + | 28917 | 0.66 | 0.599468 |
Target: 5'- -aGCgCGUGCUCgAACA-GGCuGGCGUc- -3' miRNA: 3'- gaCG-GCGCGAG-UUGUaCCG-CCGCAau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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