Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12447 | 5' | -57 | NC_003324.1 | + | 12353 | 0.7 | 0.386054 |
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Target: 5'- uUGCCGCGCUCGGCAgcauucucgUGGUaGUGa-- -3' miRNA: 3'- gACGGCGCGAGUUGU---------ACCGcCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 47724 | 0.7 | 0.377153 |
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Target: 5'- gUGCCacaGCUCGACgAUGGCGGCc--- -3' miRNA: 3'- gACGGcg-CGAGUUG-UACCGCCGcaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 23985 | 0.7 | 0.368392 |
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Target: 5'- --uUCGCGCUCGACGcgcugguagGGCGGCGg-- -3' miRNA: 3'- gacGGCGCGAGUUGUa--------CCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 36271 | 0.7 | 0.352135 |
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Target: 5'- aUGUCGCGCaacaucaguaugcugCAGCAggucGGCGGCGUg- -3' miRNA: 3'- gACGGCGCGa--------------GUUGUa---CCGCCGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 53986 | 0.71 | 0.311054 |
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Target: 5'- uUGCCGCGCUgAugAUcuuGCGGCGg-- -3' miRNA: 3'- gACGGCGCGAgUugUAc--CGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 10382 | 0.71 | 0.303439 |
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Target: 5'- aUGCaCGCgGCggAACGUGGUGGCGUa- -3' miRNA: 3'- gACG-GCG-CGagUUGUACCGCCGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 5139 | 0.74 | 0.211612 |
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Target: 5'- -gGCCGCGUUCGGguccuUGGCGGCGg-- -3' miRNA: 3'- gaCGGCGCGAGUUgu---ACCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 35014 | 0.75 | 0.185109 |
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Target: 5'- gCUGCCGCaGCagCAGCAgcGGCGGCGg-- -3' miRNA: 3'- -GACGGCG-CGa-GUUGUa-CCGCCGCaau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 40599 | 0.79 | 0.094681 |
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Target: 5'- -cGCCGCGCUCGAuCGUGGCuccaucgGGCGUg- -3' miRNA: 3'- gaCGGCGCGAGUU-GUACCG-------CCGCAau -5' |
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| 12447 | 5' | -57 | NC_003324.1 | + | 28546 | 1.07 | 0.000828 |
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Target: 5'- cCUGCCGCGCUCAACAUGGCGGCGUUAg -3' miRNA: 3'- -GACGGCGCGAGUUGUACCGCCGCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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