Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12464 | 5' | -52.4 | NC_003324.1 | + | 13214 | 0.68 | 0.801625 |
Target: 5'- gGUCUggaUCAGCUugcCGUCGGCGaAGAugCu -3' miRNA: 3'- gCGGA---AGUUGA---GCAGCCGUcUCUugG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 50912 | 0.67 | 0.811225 |
Target: 5'- cCGCCUgCAGCgauGUgGGCGuuGAACCa -3' miRNA: 3'- -GCGGAaGUUGag-CAgCCGUcuCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 21293 | 0.67 | 0.811225 |
Target: 5'- uCGCCUUCGgcaGCUCGaaagccaGGCGGuuGAGCa -3' miRNA: 3'- -GCGGAAGU---UGAGCag-----CCGUCu-CUUGg -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 29265 | 0.67 | 0.829841 |
Target: 5'- uCGCCgggcUCGcGCUCGaUCGGCGGAuuGCg -3' miRNA: 3'- -GCGGa---AGU-UGAGC-AGCCGUCUcuUGg -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 42114 | 0.67 | 0.829841 |
Target: 5'- aGCgCUUCGcACUCGUCGccaaugccgccGCGGGGAucagcaacagACCa -3' miRNA: 3'- gCG-GAAGU-UGAGCAGC-----------CGUCUCU----------UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 56212 | 0.67 | 0.829841 |
Target: 5'- uCGCCUaUC-GCUCGaUCGGCGGcGAcauCCu -3' miRNA: 3'- -GCGGA-AGuUGAGC-AGCCGUCuCUu--GG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 36252 | 0.67 | 0.847609 |
Target: 5'- uGCUg-CAGCagGUCGGCGgcgugacggauGAGAACCu -3' miRNA: 3'- gCGGaaGUUGagCAGCCGU-----------CUCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 50499 | 0.66 | 0.856153 |
Target: 5'- aCGCCcagCGcCUUG-CGGCAGGGuucACCg -3' miRNA: 3'- -GCGGaa-GUuGAGCaGCCGUCUCu--UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 38080 | 0.66 | 0.863637 |
Target: 5'- gCGCCUUCuGGCgaagcgCGUCGGCGccgugcuGGuGACCg -3' miRNA: 3'- -GCGGAAG-UUGa-----GCAGCCGU-------CUcUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 17418 | 0.66 | 0.86771 |
Target: 5'- uGCCacgUCGccCUCGUCGGUcaugacgcggagcccGGuGAACCa -3' miRNA: 3'- gCGGa--AGUu-GAGCAGCCG---------------UCuCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 39536 | 0.66 | 0.887861 |
Target: 5'- aGCCgUCGucCUCGUCGaccaCGGGGAGCg -3' miRNA: 3'- gCGGaAGUu-GAGCAGCc---GUCUCUUGg -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 18486 | 0.66 | 0.895139 |
Target: 5'- uCGCCUUCGugUgGUUccucGCAGGGu-CCg -3' miRNA: 3'- -GCGGAAGUugAgCAGc---CGUCUCuuGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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