Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12464 | 5' | -52.4 | NC_003324.1 | + | 6114 | 0.71 | 0.631256 |
Target: 5'- -cCCUaagagCAugUCGUCGGCAG-GAGCg -3' miRNA: 3'- gcGGAa----GUugAGCAGCCGUCuCUUGg -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 4323 | 0.72 | 0.543192 |
Target: 5'- uCGaCCUUCAcCUCGUCGG-AGaAGAACUu -3' miRNA: 3'- -GC-GGAAGUuGAGCAGCCgUC-UCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 53686 | 0.72 | 0.531345 |
Target: 5'- uCGUCgcgCAGCUCGUCGaGUgucaugucgcaaaGGGGGACCg -3' miRNA: 3'- -GCGGaa-GUUGAGCAGC-CG-------------UCUCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 21953 | 0.73 | 0.510053 |
Target: 5'- gGCCU----CUCGUCGGCGGcgcgaugcaccuuGGAGCCg -3' miRNA: 3'- gCGGAaguuGAGCAGCCGUC-------------UCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 5417 | 0.73 | 0.500588 |
Target: 5'- gCGCCguuggugUCGAgaUCGUCGGCGucGAGGAUCa -3' miRNA: 3'- -GCGGa------AGUUg-AGCAGCCGU--CUCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 42989 | 0.73 | 0.479841 |
Target: 5'- cCGCCaaaUUCAcGCggGUCGGCaacGGAGAACCg -3' miRNA: 3'- -GCGG---AAGU-UGagCAGCCG---UCUCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 32096 | 0.75 | 0.420319 |
Target: 5'- gCGCC-UCGGCUCGUCGccGguGAG-GCCg -3' miRNA: 3'- -GCGGaAGUUGAGCAGC--CguCUCuUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 49287 | 0.76 | 0.348336 |
Target: 5'- gGCgUUCGGaUCGUCGGCAGgaagaucgaGGAGCCg -3' miRNA: 3'- gCGgAAGUUgAGCAGCCGUC---------UCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 27096 | 0.77 | 0.323706 |
Target: 5'- aGCCUgcgccaaggacUCGAgUCGUgGGCAGAGA-CCg -3' miRNA: 3'- gCGGA-----------AGUUgAGCAgCCGUCUCUuGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 8324 | 0.79 | 0.220715 |
Target: 5'- aCGCCgUCGGCgcucguuacggugacCGUCGGCGGGGAGCUg -3' miRNA: 3'- -GCGGaAGUUGa--------------GCAGCCGUCUCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 18223 | 0.8 | 0.19247 |
Target: 5'- uCGCCUUCuuUUCGUCGGCGGucAGGuuGCCa -3' miRNA: 3'- -GCGGAAGuuGAGCAGCCGUC--UCU--UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 39489 | 1.12 | 0.001554 |
Target: 5'- uCGCCUUCAACUCGUCGGCAGAGAACCc -3' miRNA: 3'- -GCGGAAGUUGAGCAGCCGUCUCUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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