Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12464 | 5' | -52.4 | NC_003324.1 | + | 49541 | 0.7 | 0.653468 |
Target: 5'- gGCauagUC--CUCGUCGGCAGAGGugauauucuGCCa -3' miRNA: 3'- gCGga--AGuuGAGCAGCCGUCUCU---------UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 8671 | 0.7 | 0.675599 |
Target: 5'- gCGCCcgUGACgCGguccUCGGCAGuGAGCCa -3' miRNA: 3'- -GCGGaaGUUGaGC----AGCCGUCuCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 38120 | 0.69 | 0.751135 |
Target: 5'- cCGCCgucUCGugUCgGUCGGCGGAauguuGAcgcGCCc -3' miRNA: 3'- -GCGGa--AGUugAG-CAGCCGUCU-----CU---UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 49605 | 0.68 | 0.761527 |
Target: 5'- uCGCCUcgCcGCUCGauaCGGCcGAGAguGCCa -3' miRNA: 3'- -GCGGAa-GuUGAGCa--GCCGuCUCU--UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 43369 | 0.68 | 0.761527 |
Target: 5'- gCGCCUgCGAggCGUCGGCGaaauccuuGAGAaacuGCCg -3' miRNA: 3'- -GCGGAaGUUgaGCAGCCGU--------CUCU----UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 16740 | 0.68 | 0.771783 |
Target: 5'- uGCCUcCGACUCGa-GGCAGAGcgUg -3' miRNA: 3'- gCGGAaGUUGAGCagCCGUCUCuuGg -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 13214 | 0.68 | 0.801625 |
Target: 5'- gGUCUggaUCAGCUugcCGUCGGCGaAGAugCu -3' miRNA: 3'- gCGGA---AGUUGA---GCAGCCGUcUCUugG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 50912 | 0.67 | 0.811225 |
Target: 5'- cCGCCUgCAGCgauGUgGGCGuuGAACCa -3' miRNA: 3'- -GCGGAaGUUGag-CAgCCGUcuCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 21293 | 0.67 | 0.811225 |
Target: 5'- uCGCCUUCGgcaGCUCGaaagccaGGCGGuuGAGCa -3' miRNA: 3'- -GCGGAAGU---UGAGCag-----CCGUCu-CUUGg -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 36252 | 0.67 | 0.847609 |
Target: 5'- uGCUg-CAGCagGUCGGCGgcgugacggauGAGAACCu -3' miRNA: 3'- gCGGaaGUUGagCAGCCGU-----------CUCUUGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 50499 | 0.66 | 0.856153 |
Target: 5'- aCGCCcagCGcCUUG-CGGCAGGGuucACCg -3' miRNA: 3'- -GCGGaa-GUuGAGCaGCCGUCUCu--UGG- -5' |
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12464 | 5' | -52.4 | NC_003324.1 | + | 18486 | 0.66 | 0.895139 |
Target: 5'- uCGCCUUCGugUgGUUccucGCAGGGu-CCg -3' miRNA: 3'- -GCGGAAGUugAgCAGc---CGUCUCuuGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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