Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12465 | 3' | -50.4 | NC_003324.1 | + | 3246 | 0.68 | 0.878278 |
Target: 5'- aUCGGCG-CGGUGCCGGugcuuUCGGa -3' miRNA: 3'- gAGCUGUaGCUACGGCUuccuuAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 33794 | 0.68 | 0.885934 |
Target: 5'- gCUCGAguCAUCGucaCCGAAGG-AUUGGa -3' miRNA: 3'- -GAGCU--GUAGCuacGGCUUCCuUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 22973 | 0.68 | 0.885934 |
Target: 5'- aUCGGCAUgGAUGCUGAguaugAGGca-CGGu -3' miRNA: 3'- gAGCUGUAgCUACGGCU-----UCCuuaGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 23770 | 0.67 | 0.911898 |
Target: 5'- gUCGACAUCGAUgugaagcccggcgcGCCGu-GGAuacCGGc -3' miRNA: 3'- gAGCUGUAGCUA--------------CGGCuuCCUua-GCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 5397 | 0.67 | 0.920111 |
Target: 5'- gUCGGCGUCGAggaucaUGUCGAu-GAcgCGGg -3' miRNA: 3'- gAGCUGUAGCU------ACGGCUucCUuaGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 32704 | 0.67 | 0.926103 |
Target: 5'- cCUCGACAgUGAUGUCGAGuGGAGc--- -3' miRNA: 3'- -GAGCUGUaGCUACGGCUU-CCUUagcc -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 54343 | 0.67 | 0.931809 |
Target: 5'- gCUCGGCGcUGAUGCCGGuccauGGcAAcCGGu -3' miRNA: 3'- -GAGCUGUaGCUACGGCUu----CC-UUaGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 41836 | 0.67 | 0.93723 |
Target: 5'- gUCGACAUCGAgcaaaugGuuGggGGugccgUGGc -3' miRNA: 3'- gAGCUGUAGCUa------CggCuuCCuua--GCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 40204 | 0.78 | 0.387336 |
Target: 5'- uUCGGCAUCGAUGUCGAGacgcuGGAAUggUGGa -3' miRNA: 3'- gAGCUGUAGCUACGGCUU-----CCUUA--GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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