Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12467 | 5' | -52.7 | NC_003324.1 | + | 29989 | 0.66 | 0.837032 |
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Target: 5'- --cGCCguccagcaugGCCAGCAGCGC--GCCGAAg -3' miRNA: 3'- ccaUGGa---------CGGUUGUUGCGcaCGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 29101 | 0.66 | 0.837032 |
|
Target: 5'- cGGUACCgcGUCGA--GCGCGcUGCUAAGg -3' miRNA: 3'- -CCAUGGa-CGGUUguUGCGC-ACGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 39261 | 0.66 | 0.846017 |
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Target: 5'- cGGUugCcGCCGugGcuGCGCcagaagGUGCCGAu -3' miRNA: 3'- -CCAugGaCGGUugU--UGCG------CACGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 14500 | 0.66 | 0.827819 |
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Target: 5'- cGGUGCa-GCCAACAcuGCGCG-GCa--- -3' miRNA: 3'- -CCAUGgaCGGUUGU--UGCGCaCGguuu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 42175 | 0.66 | 0.818388 |
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Target: 5'- cGUACUUGCCgAACGACGgcacUGcGCCGGAg -3' miRNA: 3'- cCAUGGACGG-UUGUUGC----GCaCGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 166 | 0.66 | 0.808749 |
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Target: 5'- gGGgauauuCCUGUaGACGGCGCGcGCCAu- -3' miRNA: 3'- -CCau----GGACGgUUGUUGCGCaCGGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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