Results 1 - 20 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12469 | 5' | -52.7 | NC_003324.1 | + | 17168 | 0.66 | 0.896479 |
Target: 5'- aGCGAUUGacgaggcGCUggcgcgucUGUCGGACG-CGGCu -3' miRNA: 3'- gCGCUAGCa------UGA--------ACGGCUUGCuGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 11567 | 0.66 | 0.894361 |
Target: 5'- gGCGAgcagcCGaUGCUgcucccgcggcuccUGCCGGagacugguGCGACGGCn -3' miRNA: 3'- gCGCUa----GC-AUGA--------------ACGGCU--------UGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 13305 | 0.66 | 0.887128 |
Target: 5'- -aCGAUCGggac-GCCGAAaagcuugaggugcuCGACGGCa -3' miRNA: 3'- gcGCUAGCaugaaCGGCUU--------------GCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 2679 | 0.66 | 0.884908 |
Target: 5'- gCGCGGUgGccGCUucggcaagggcacgaUGUCG-GCGGCGGCa -3' miRNA: 3'- -GCGCUAgCa-UGA---------------ACGGCuUGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 49027 | 0.66 | 0.881911 |
Target: 5'- uGCcg-CG-AC-UGCCGAagaaGCGACGGCc -3' miRNA: 3'- gCGcuaGCaUGaACGGCU----UGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 21910 | 0.66 | 0.881911 |
Target: 5'- gCGCGA----ACUgaUGCagaaggCGGACGACGGCg -3' miRNA: 3'- -GCGCUagcaUGA--ACG------GCUUGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 5349 | 0.66 | 0.874239 |
Target: 5'- aGCGAgaUCGaggauaucGCcUGCCGcugguGCGGCGGCc -3' miRNA: 3'- gCGCU--AGCa-------UGaACGGCu----UGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 51630 | 0.66 | 0.874239 |
Target: 5'- uGCGAaCGccgUUGCCGcaaGACGGCa -3' miRNA: 3'- gCGCUaGCaugAACGGCuugCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 19964 | 0.66 | 0.874239 |
Target: 5'- gCGCGuUgGUugUgaaGUCGAuuAUGGCGGCg -3' miRNA: 3'- -GCGCuAgCAugAa--CGGCU--UGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 27836 | 0.66 | 0.874239 |
Target: 5'- cCGCuGGU-GUAgUUGUCGAGguaGGCGGCg -3' miRNA: 3'- -GCG-CUAgCAUgAACGGCUUg--CUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 30526 | 0.66 | 0.874239 |
Target: 5'- gGCGAccuUCGcUGCcgcUGCCGcuGCGACGcGCu -3' miRNA: 3'- gCGCU---AGC-AUGa--ACGGCu-UGCUGC-CG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 18491 | 0.66 | 0.866318 |
Target: 5'- uGCGAgcUCGU---UGgCGAGCGucACGGCg -3' miRNA: 3'- gCGCU--AGCAugaACgGCUUGC--UGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 41322 | 0.66 | 0.866318 |
Target: 5'- uCGCGAUC--ACgaUGUCGAACGGCcucgaaaucGGCg -3' miRNA: 3'- -GCGCUAGcaUGa-ACGGCUUGCUG---------CCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 27641 | 0.66 | 0.866318 |
Target: 5'- uCGCGGaucUCGaaccGCUUGCCGuucGCGACcaccucaugcguGGCg -3' miRNA: 3'- -GCGCU---AGCa---UGAACGGCu--UGCUG------------CCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 17799 | 0.66 | 0.866318 |
Target: 5'- gGCGAUCugGUAgcgUGCCucACGGCGcGCa -3' miRNA: 3'- gCGCUAG--CAUga-ACGGcuUGCUGC-CG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 49972 | 0.66 | 0.858153 |
Target: 5'- gGCGAUCGgcGC-UGCCcuGAUGGgGGCa -3' miRNA: 3'- gCGCUAGCa-UGaACGGc-UUGCUgCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 11348 | 0.66 | 0.855657 |
Target: 5'- aCGCGGUCGcUGauguugucaaucagUUUGCCGca-GAUGGCu -3' miRNA: 3'- -GCGCUAGC-AU--------------GAACGGCuugCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 23261 | 0.67 | 0.849752 |
Target: 5'- aGCGGUacCGcGCUcGCCGAccauacCGugGGCg -3' miRNA: 3'- gCGCUA--GCaUGAaCGGCUu-----GCugCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 19547 | 0.67 | 0.849752 |
Target: 5'- aCGCGcAUa-UACUcGCCGA-CGGCGGUc -3' miRNA: 3'- -GCGC-UAgcAUGAaCGGCUuGCUGCCG- -5' |
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12469 | 5' | -52.7 | NC_003324.1 | + | 1888 | 0.67 | 0.841123 |
Target: 5'- cCGU--UCGUGCUU-CCGAACGguaGGCa -3' miRNA: 3'- -GCGcuAGCAUGAAcGGCUUGCug-CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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