Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12480 | 3' | -63.2 | NC_003324.1 | + | 25315 | 0.7 | 0.166383 |
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Target: 5'- uAAGGGCgGCCAggugcucgaACCGUcaGUCGGCG-CAGg -3' miRNA: 3'- -UUCCCG-CGGU---------UGGCG--CAGCCGCgGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 1567 | 0.71 | 0.144758 |
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Target: 5'- aAAGGGCGCCAGcCCGCGaUCaccaaucucgacaaGGCGCgCGa -3' miRNA: 3'- -UUCCCGCGGUU-GGCGC-AG--------------CCGCG-GUc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 23126 | 0.72 | 0.12439 |
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Target: 5'- uGGGGCGCCGAUUuCGUCaagcucuacccgGGCGCCAa -3' miRNA: 3'- uUCCCGCGGUUGGcGCAG------------CCGCGGUc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 45254 | 0.73 | 0.108745 |
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Target: 5'- cGAGGaGCGCCaAAUCGCGUCcuacuucgaacGCGCCAGg -3' miRNA: 3'- -UUCC-CGCGG-UUGGCGCAGc----------CGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48060 | 0.73 | 0.103019 |
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Target: 5'- -cGGGUcaGUCAG-CGCGUCGGUGCCGGu -3' miRNA: 3'- uuCCCG--CGGUUgGCGCAGCCGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 23497 | 0.73 | 0.103019 |
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Target: 5'- cAGGGCGCC-ACCGCG-CGGCcaacgagaaaGCCGa -3' miRNA: 3'- uUCCCGCGGuUGGCGCaGCCG----------CGGUc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 16321 | 0.73 | 0.100264 |
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Target: 5'- uAGGGcccuGCGCUGGCCGCGUCGGCacgGCCu- -3' miRNA: 3'- -UUCC----CGCGGUUGGCGCAGCCG---CGGuc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48722 | 1.05 | 0.000345 |
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Target: 5'- aAAGGGCGCCAACCGCGUCGGCGCCAGc -3' miRNA: 3'- -UUCCCGCGGUUGGCGCAGCCGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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