Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12480 | 3' | -63.2 | NC_003324.1 | + | 38073 | 0.69 | 0.194323 |
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Target: 5'- -cGGuacuGCGCCuucuggcgAAgCGCGUCGGCGCCGu -3' miRNA: 3'- uuCC----CGCGG--------UUgGCGCAGCCGCGGUc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 20514 | 0.68 | 0.215161 |
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Target: 5'- cGAGGGCgugcccGCCGACCGCaagcgcccaUCaGCGCCGGc -3' miRNA: 3'- -UUCCCG------CGGUUGGCGc--------AGcCGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 29955 | 0.68 | 0.226283 |
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Target: 5'- gAGGGGCGUC--CUGCGUagcggCGGCaGCCGGg -3' miRNA: 3'- -UUCCCGCGGuuGGCGCA-----GCCG-CGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 19487 | 0.66 | 0.296345 |
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Target: 5'- -uGGGCGaua---GCGUCGGCGUCGGc -3' miRNA: 3'- uuCCCGCgguuggCGCAGCCGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 26024 | 0.66 | 0.325709 |
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Target: 5'- uGAGcGCGCCAAUgGCGUCaccGGUcuGCCGGc -3' miRNA: 3'- -UUCcCGCGGUUGgCGCAG---CCG--CGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 18806 | 0.66 | 0.325709 |
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Target: 5'- -cGGGU-CCAuggcGCgGCGaaggUCGGCGCCAGu -3' miRNA: 3'- uuCCCGcGGU----UGgCGC----AGCCGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 28642 | 0.66 | 0.333379 |
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Target: 5'- -cGGGcCGCCuuguCgCGCGUCguGGUGCCAu -3' miRNA: 3'- uuCCC-GCGGuu--G-GCGCAG--CCGCGGUc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48722 | 1.05 | 0.000345 |
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Target: 5'- aAAGGGCGCCAACCGCGUCGGCGCCAGc -3' miRNA: 3'- -UUCCCGCGGUUGGCGCAGCCGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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