Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12481 | 3' | -55.5 | NC_003324.1 | + | 55286 | 0.67 | 0.644046 |
Target: 5'- gCGAucagucauuGGGCACCAUCGACAuGgCgugggCGAg -3' miRNA: 3'- gGCU---------UCCGUGGUAGCUGU-CgGa----GCUa -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 44682 | 0.67 | 0.665939 |
Target: 5'- gUCGAucGUGgUGUUGGCAGCCUCGAc -3' miRNA: 3'- -GGCUucCGUgGUAGCUGUCGGAGCUa -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 30563 | 0.67 | 0.676844 |
Target: 5'- uCCGAGGGC-CUGcUCGGCGGCgC-CGAUu -3' miRNA: 3'- -GGCUUCCGuGGU-AGCUGUCG-GaGCUA- -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 1482 | 0.66 | 0.687706 |
Target: 5'- gCGAAGaacgcccaGCACCGUCGGC-GCaUCGAUg -3' miRNA: 3'- gGCUUC--------CGUGGUAGCUGuCGgAGCUA- -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 48575 | 0.66 | 0.709261 |
Target: 5'- aUGAGGGCAaggacagcggCGUCG-CAGCgCUCGAUg -3' miRNA: 3'- gGCUUCCGUg---------GUAGCuGUCG-GAGCUA- -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 44314 | 0.66 | 0.709261 |
Target: 5'- gCGguGGCGCgGcCGAUAGCCUCu-- -3' miRNA: 3'- gGCuuCCGUGgUaGCUGUCGGAGcua -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 50190 | 0.66 | 0.709261 |
Target: 5'- gCCauGGGCAUCAgcgacaagUCGGCgAGCCUUGAa -3' miRNA: 3'- -GGcuUCCGUGGU--------AGCUG-UCGGAGCUa -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 30384 | 0.66 | 0.719931 |
Target: 5'- uCCGGcgucgcuacAGGCAUguUCGGCGGCUUgGGc -3' miRNA: 3'- -GGCU---------UCCGUGguAGCUGUCGGAgCUa -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 38943 | 0.66 | 0.719931 |
Target: 5'- -aGuAGGCGCCGucUCGACccuuGGCCgUCGAUu -3' miRNA: 3'- ggCuUCCGUGGU--AGCUG----UCGG-AGCUA- -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 29362 | 0.66 | 0.730515 |
Target: 5'- cCUGAuaGGGCuuGCCcUCGucggugacgacaACGGCCUCGGUa -3' miRNA: 3'- -GGCU--UCCG--UGGuAGC------------UGUCGGAGCUA- -5' |
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12481 | 3' | -55.5 | NC_003324.1 | + | 41456 | 0.66 | 0.730515 |
Target: 5'- aCCGAAGGCGgCAUUGGuaaggggacgcgUAGCCaCGGg -3' miRNA: 3'- -GGCUUCCGUgGUAGCU------------GUCGGaGCUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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