Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12483 | 3' | -58.4 | NC_003324.1 | + | 7408 | 0.67 | 0.528214 |
Target: 5'- gCUCGACcaauggGGCACggagauuggcaaggGUgCUGCUGCCCg -3' miRNA: 3'- gGGGCUG------CCGUGaa------------UAgGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 12897 | 0.66 | 0.607693 |
Target: 5'- cCCCUuGCGGCGCgUcgaaacgcuggaugGUUuuGCUGCCa -3' miRNA: 3'- -GGGGcUGCCGUGaA--------------UAGggCGACGGg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 24668 | 0.66 | 0.589697 |
Target: 5'- gCCCCGAUGauccuGCGCUggucgCCCGCaGCg- -3' miRNA: 3'- -GGGGCUGC-----CGUGAaua--GGGCGaCGgg -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 18288 | 0.66 | 0.610876 |
Target: 5'- -gCCGAC-GCGCgggcaaUCGCUGCCCc -3' miRNA: 3'- ggGGCUGcCGUGaauag-GGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 21458 | 0.66 | 0.579153 |
Target: 5'- aUCCGGaucuuCGGCAUagagCUCGUUGCCCu -3' miRNA: 3'- gGGGCU-----GCCGUGaauaGGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 42182 | 0.66 | 0.579153 |
Target: 5'- gCCgaaCGACGGCACUgcgCCggaGUgaucgGCCCa -3' miRNA: 3'- -GGg--GCUGCCGUGAauaGGg--CGa----CGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 4286 | 0.66 | 0.568651 |
Target: 5'- uCCgCCGGUGGCAagaUGUCCCGCaagcGCaCCg -3' miRNA: 3'- -GG-GGCUGCCGUga-AUAGGGCGa---CG-GG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 28589 | 0.66 | 0.568651 |
Target: 5'- uUuuGGCGGUucgcgGCUUccgCCCGCUgucGCCCu -3' miRNA: 3'- gGggCUGCCG-----UGAAua-GGGCGA---CGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 29278 | 0.66 | 0.558196 |
Target: 5'- gCUCGAuCGGCGgaUUGcguuccagcUCCUGCUGCUCg -3' miRNA: 3'- gGGGCU-GCCGUg-AAU---------AGGGCGACGGG- -5' |
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12483 | 3' | -58.4 | NC_003324.1 | + | 49087 | 0.66 | 0.558196 |
Target: 5'- -gCCGaaaGCGGCGucUAUUUCGCUGCCg -3' miRNA: 3'- ggGGC---UGCCGUgaAUAGGGCGACGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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