Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12486 | 3' | -51.7 | NC_003324.1 | + | 27672 | 0.66 | 0.903511 |
Target: 5'- cCACCUCAuGCGuggcGCCGAgCUucUCCUUGg -3' miRNA: 3'- -GUGGGGUuUGUuu--CGGCU-GA--AGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 30814 | 0.66 | 0.896465 |
Target: 5'- uCACuCCCGAGCAGcaggcGGCCGACg-CCa-- -3' miRNA: 3'- -GUG-GGGUUUGUU-----UCGGCUGaaGGagc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 17729 | 0.66 | 0.896465 |
Target: 5'- cCGCgCC-AACGucGUCGAUaUCCUCGg -3' miRNA: 3'- -GUGgGGuUUGUuuCGGCUGaAGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 40186 | 0.66 | 0.896465 |
Target: 5'- aCAUCagCCGAGCGAuGCCGACgaagcgcUUCUCGa -3' miRNA: 3'- -GUGG--GGUUUGUUuCGGCUGa------AGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 49146 | 0.66 | 0.889141 |
Target: 5'- gGCCCCGGuGCAGAGCCGGauggauugCCcCGc -3' miRNA: 3'- gUGGGGUU-UGUUUCGGCUgaa-----GGaGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 23142 | 0.66 | 0.889141 |
Target: 5'- cCACCUCGuggGACAGuggggcGCCGAUUUCgUCa -3' miRNA: 3'- -GUGGGGU---UUGUUu-----CGGCUGAAGgAGc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 5417 | 0.66 | 0.881543 |
Target: 5'- gCGCCCgCAccuGCGgcGCCGAgUUCUUCu -3' miRNA: 3'- -GUGGG-GUu--UGUuuCGGCUgAAGGAGc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 35516 | 0.66 | 0.873677 |
Target: 5'- gAUCCUgcACGAAGCCGGgUUCgUCu -3' miRNA: 3'- gUGGGGuuUGUUUCGGCUgAAGgAGc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 7382 | 0.66 | 0.873677 |
Target: 5'- uCugCCCGcACGcuGcCCGGCUUCCUg- -3' miRNA: 3'- -GugGGGUuUGUuuC-GGCUGAAGGAgc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 32344 | 0.67 | 0.86555 |
Target: 5'- cCugCCUAGcgGCAucGcCCGGCUUCCUg- -3' miRNA: 3'- -GugGGGUU--UGUuuC-GGCUGAAGGAgc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 38191 | 0.67 | 0.863061 |
Target: 5'- aCGCCCCAcaagaacggucaccAGCAcggcGCCGACgcgCUUCGc -3' miRNA: 3'- -GUGGGGU--------------UUGUuu--CGGCUGaa-GGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 1322 | 0.67 | 0.857168 |
Target: 5'- aCGCCUCcGACGu-GCuCGACUcCCUCGu -3' miRNA: 3'- -GUGGGGuUUGUuuCG-GCUGAaGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 55499 | 0.67 | 0.848539 |
Target: 5'- cCACgCCGGGCAgGAGCCuGCcggCCUCGa -3' miRNA: 3'- -GUGgGGUUUGU-UUCGGcUGaa-GGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 25984 | 0.67 | 0.839674 |
Target: 5'- gCGCCCCAGACcagaucgauAGGcCCGACUUCg--- -3' miRNA: 3'- -GUGGGGUUUGu--------UUC-GGCUGAAGgagc -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 8481 | 0.67 | 0.839674 |
Target: 5'- aCGCCCCGAugGuGGCCGgaGCUga-UCGa -3' miRNA: 3'- -GUGGGGUUugUuUCGGC--UGAaggAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 27989 | 0.68 | 0.821268 |
Target: 5'- gACCCCGcACGuGGCCG-CUUCgaCGa -3' miRNA: 3'- gUGGGGUuUGUuUCGGCuGAAGgaGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 20165 | 0.68 | 0.792127 |
Target: 5'- gAUCCaGAACGcuGCCGGCagCCUCGg -3' miRNA: 3'- gUGGGgUUUGUuuCGGCUGaaGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 5954 | 0.68 | 0.782049 |
Target: 5'- uCAUCCC---CAAGGCCGGgugcggUUUCCUCGa -3' miRNA: 3'- -GUGGGGuuuGUUUCGGCU------GAAGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 39656 | 0.69 | 0.761416 |
Target: 5'- aGCCaCCGAGCGc-GCCcuCUUCCUCGa -3' miRNA: 3'- gUGG-GGUUUGUuuCGGcuGAAGGAGC- -5' |
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12486 | 3' | -51.7 | NC_003324.1 | + | 48932 | 0.69 | 0.761416 |
Target: 5'- gACCUgGu-CAAGGCCGucGCUUCUUCGg -3' miRNA: 3'- gUGGGgUuuGUUUCGGC--UGAAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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