Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12490 | 5' | -58.3 | NC_003324.1 | + | 31036 | 0.66 | 0.554637 |
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Target: 5'- cCGGCGCGGUcGGcGUucaacuccuGCUgacgCGCUGCg -3' miRNA: 3'- aGUCGCGCCGuUCaCG---------CGAa---GCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 40429 | 0.66 | 0.554637 |
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Target: 5'- gCAGCGaaagcCGGCAAGacUGCuGCagCGCCGa -3' miRNA: 3'- aGUCGC-----GCCGUUC--ACG-CGaaGCGGCg -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 31093 | 0.66 | 0.554637 |
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Target: 5'- cCGGUccuuGCGGCAA-UGCGCU--GCUGCu -3' miRNA: 3'- aGUCG----CGCCGUUcACGCGAagCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 5981 | 0.66 | 0.56206 |
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Target: 5'- gCAGCGCGaGCAcGGUGgaggagaucgaagcCGCaaUUGCCGCc -3' miRNA: 3'- aGUCGCGC-CGU-UCAC--------------GCGa-AGCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 23232 | 0.66 | 0.56525 |
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Target: 5'- -gGGCGcCGGCAAGa-CGUUU-GCCGCc -3' miRNA: 3'- agUCGC-GCCGUUCacGCGAAgCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 7660 | 0.66 | 0.56525 |
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Target: 5'- gCAGUGcCGGCug--GCGUUuaugugUCGCUGCa -3' miRNA: 3'- aGUCGC-GCCGuucaCGCGA------AGCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 42380 | 0.66 | 0.575914 |
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Target: 5'- cCGGCacGUGGCAGGcgGCGCacucagcgUUGCCGa -3' miRNA: 3'- aGUCG--CGCCGUUCa-CGCGa-------AGCGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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