Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12490 | 5' | -58.3 | NC_003324.1 | + | 56089 | 0.73 | 0.204411 |
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Target: 5'- aCGGCGCGcuccgcccuGUggGUGCGCUucucgacgaUCGCgCGCa -3' miRNA: 3'- aGUCGCGC---------CGuuCACGCGA---------AGCG-GCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 19493 | 0.73 | 0.199126 |
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Target: 5'- aUAGCGuCGGCGucGGcGUGCUUCuuGCCGCg -3' miRNA: 3'- aGUCGC-GCCGU--UCaCGCGAAG--CGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 31321 | 0.74 | 0.193961 |
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Target: 5'- gCAGUGCGGCcGGcgGCGCUggCGCUGg -3' miRNA: 3'- aGUCGCGCCGuUCa-CGCGAa-GCGGCg -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 5496 | 0.75 | 0.165377 |
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Target: 5'- cUCGGCGCcGCAGGUGCGggcgccauCUuccgagccgaaaUCGCCGCu -3' miRNA: 3'- -AGUCGCGcCGUUCACGC--------GA------------AGCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 26799 | 0.77 | 0.109831 |
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Target: 5'- gCAGaCGCGGCc-GUGCGCUUggcuggcuuuggCGCCGCu -3' miRNA: 3'- aGUC-GCGCCGuuCACGCGAA------------GCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 51415 | 0.77 | 0.106828 |
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Target: 5'- aUCAGcCGCGGCGaccggauuugGGUGCGCgagaaCGCUGCu -3' miRNA: 3'- -AGUC-GCGCCGU----------UCACGCGaa---GCGGCG- -5' |
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| 12490 | 5' | -58.3 | NC_003324.1 | + | 53917 | 1.11 | 0.000367 |
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Target: 5'- aUCAGCGCGGCAAGUGCGCUUCGCCGCc -3' miRNA: 3'- -AGUCGCGCCGUUCACGCGAAGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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