Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12493 | 5' | -53.8 | NC_003324.1 | + | 16432 | 0.7 | 0.58968 |
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Target: 5'- gGUC-CUGGAcgACGCGAUCGAcggcaacgccGuCGCGGa -3' miRNA: 3'- -UAGcGACCU--UGCGCUAGUU----------CuGCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 57038 | 0.68 | 0.677916 |
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Target: 5'- --gGCUGGGgaGCgGCGGcgAGGGCGCGGa -3' miRNA: 3'- uagCGACCU--UG-CGCUagUUCUGCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 17039 | 0.66 | 0.783494 |
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Target: 5'- -gCGCUGGGuaaggaacuACGCGAUCugcugGCGGa -3' miRNA: 3'- uaGCGACCU---------UGCGCUAGuucugCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 50926 | 0.66 | 0.793369 |
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Target: 5'- uGUCGCUGGGcgaaccgccuGCaGCGAUguGGGCGUu- -3' miRNA: 3'- -UAGCGACCU----------UG-CGCUAguUCUGCGcc -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 30631 | 0.66 | 0.793369 |
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Target: 5'- -cCGCUGGAguagagcaGCGCGucgCAGcGGCaGCGGc -3' miRNA: 3'- uaGCGACCU--------UGCGCua-GUU-CUG-CGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 23028 | 0.66 | 0.8126 |
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Target: 5'- cAUCGCcaccggagacUGGGACGCGGUUAuuGuCGCGc -3' miRNA: 3'- -UAGCG----------ACCUUGCGCUAGUu-CuGCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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