Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
12493 | 5' | -53.8 | NC_003324.1 | + | 48763 | 0.71 | 0.503633 |
Target: 5'- -cUGCUGGAAgGUGAUgcCGAGAUGCGc -3' miRNA: 3'- uaGCGACCUUgCGCUA--GUUCUGCGCc -5' |
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12493 | 5' | -53.8 | NC_003324.1 | + | 49105 | 0.72 | 0.462647 |
Target: 5'- uUCGCUGccgguGACGCGAUCGccaAGAUgGCGGg -3' miRNA: 3'- uAGCGACc----UUGCGCUAGU---UCUG-CGCC- -5' |
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12493 | 5' | -53.8 | NC_003324.1 | + | 29361 | 0.73 | 0.433088 |
Target: 5'- --aGCUGGAACGCaauccgccGAUCGAG-CGCGa -3' miRNA: 3'- uagCGACCUUGCG--------CUAGUUCuGCGCc -5' |
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12493 | 5' | -53.8 | NC_003324.1 | + | 28839 | 0.77 | 0.234886 |
Target: 5'- --aGCUGGu-CGCGGUCAgAGAUGCGGa -3' miRNA: 3'- uagCGACCuuGCGCUAGU-UCUGCGCC- -5' |
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12493 | 5' | -53.8 | NC_003324.1 | + | 17114 | 0.83 | 0.097273 |
Target: 5'- -aCGCUGcAGCGCucGAUCAAGACGCGGg -3' miRNA: 3'- uaGCGACcUUGCG--CUAGUUCUGCGCC- -5' |
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12493 | 5' | -53.8 | NC_003324.1 | + | 55341 | 1.08 | 0.001822 |
Target: 5'- gAUCGCUGGAACGCGAUCAAGACGCGGc -3' miRNA: 3'- -UAGCGACCUUGCGCUAGUUCUGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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