Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12493 | 5' | -53.8 | NC_003324.1 | + | 23028 | 0.66 | 0.8126 |
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Target: 5'- cAUCGCcaccggagacUGGGACGCGGUUAuuGuCGCGc -3' miRNA: 3'- -UAGCG----------ACCUUGCGCUAGUu-CuGCGCc -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 17423 | 0.66 | 0.8126 |
|
Target: 5'- cGUCGCccu--CGuCGGUCAuGACGCGGa -3' miRNA: 3'- -UAGCGaccuuGC-GCUAGUuCUGCGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 7767 | 0.66 | 0.803075 |
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Target: 5'- cUCGCagGGAgGCGCGAUCGcaacGGGCuuugccgccGCGGc -3' miRNA: 3'- uAGCGa-CCU-UGCGCUAGU----UCUG---------CGCC- -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 40612 | 0.66 | 0.783494 |
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Target: 5'- uUCGCUGGGGCcauccGCcgGAUCAGGGCGa-- -3' miRNA: 3'- uAGCGACCUUG-----CG--CUAGUUCUGCgcc -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 29867 | 0.66 | 0.7805 |
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Target: 5'- cGUCGUgacaucaggcagggUGGAuacgGCGCGAUCuucGGCGCGc -3' miRNA: 3'- -UAGCG--------------ACCU----UGCGCUAGuu-CUGCGCc -5' |
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| 12493 | 5' | -53.8 | NC_003324.1 | + | 24881 | 0.66 | 0.8126 |
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Target: 5'- cUCGUUGGAAC-CG-UCGAGgaaGCGGu -3' miRNA: 3'- uAGCGACCUUGcGCuAGUUCug-CGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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