Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12496 | 5' | -53.5 | NC_003324.1 | + | 512 | 0.68 | 0.689309 |
Target: 5'- gAUCACCAAGGucGGCAGcUCGAccaucuaCCGCu -3' miRNA: 3'- -UAGUGGUUCCu-UCGUU-AGCUcc-----GGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 1409 | 0.66 | 0.785404 |
Target: 5'- uUCACgAGGGAgucgAGCAcGUCGgAGGCgUGCu -3' miRNA: 3'- uAGUGgUUCCU----UCGU-UAGC-UCCG-GCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 3219 | 0.67 | 0.720116 |
Target: 5'- cUCGCCAAGGGGcGCGucgaucacgccuUUGAGGCagGCg -3' miRNA: 3'- uAGUGGUUCCUU-CGUu-----------AGCUCCGg-CG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 5990 | 0.66 | 0.795412 |
Target: 5'- -gCACgGuGGAGGaGAUCGAaGCCGCa -3' miRNA: 3'- uaGUGgUuCCUUCgUUAGCUcCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 13178 | 0.67 | 0.722292 |
Target: 5'- cGUCAgCGAGGcugcgucggcGGCGGUCGGcGGCgGCg -3' miRNA: 3'- -UAGUgGUUCCu---------UCGUUAGCU-CCGgCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 14297 | 0.67 | 0.750208 |
Target: 5'- uUCAUCAAGGAagcgaccaacuacGGCAGUgaagcacgucagagCGAGGCaGCu -3' miRNA: 3'- uAGUGGUUCCU-------------UCGUUA--------------GCUCCGgCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 14894 | 0.73 | 0.4103 |
Target: 5'- -cCGCCcAGGAAGCugcCGAugccGGCCGCg -3' miRNA: 3'- uaGUGGuUCCUUCGuuaGCU----CCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 18382 | 0.68 | 0.677069 |
Target: 5'- cUCACCAAGGccgGGGCAG-CGAuugcccgcgcgucGGCCGa -3' miRNA: 3'- uAGUGGUUCC---UUCGUUaGCU-------------CCGGCg -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 19938 | 0.67 | 0.733116 |
Target: 5'- --gACCAGGGAcggaAGCAguAUCGAaGGCgCGUu -3' miRNA: 3'- uagUGGUUCCU----UCGU--UAGCU-CCG-GCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 21380 | 0.67 | 0.722292 |
Target: 5'- uAUCGCCGgcgAGGcGGCGAaCGcGGCCGa -3' miRNA: 3'- -UAGUGGU---UCCuUCGUUaGCuCCGGCg -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 21929 | 0.69 | 0.633343 |
Target: 5'- -cCGCCGAGGAGGCcc----GGCUGCg -3' miRNA: 3'- uaGUGGUUCCUUCGuuagcuCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 24574 | 0.71 | 0.479054 |
Target: 5'- cGUCACCGcugcGGGcgaccAGCGcaggaucAUCGGGGCCGUa -3' miRNA: 3'- -UAGUGGU----UCCu----UCGU-------UAGCUCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 26551 | 0.7 | 0.577331 |
Target: 5'- --uGCCGAGGucGAGCAAUUGAuGUCGCc -3' miRNA: 3'- uagUGGUUCC--UUCGUUAGCUcCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 27347 | 0.67 | 0.743834 |
Target: 5'- -cCACCAAcgcGGAAGCcg-UGuucGGCCGCu -3' miRNA: 3'- uaGUGGUU---CCUUCGuuaGCu--CCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 27765 | 0.7 | 0.581784 |
Target: 5'- cAUCGCCAAGGAgaAGC--UCGGcgccacgcaugagguGGUCGCg -3' miRNA: 3'- -UAGUGGUUCCU--UCGuuAGCU---------------CCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 29081 | 0.72 | 0.449434 |
Target: 5'- --uGCUAAGGAAGCGAaaUCGAcGCUGCc -3' miRNA: 3'- uagUGGUUCCUUCGUU--AGCUcCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 29234 | 0.66 | 0.785404 |
Target: 5'- cUCACCAAGacGGCGGcuguUCG-GGCCuGCa -3' miRNA: 3'- uAGUGGUUCcuUCGUU----AGCuCCGG-CG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 29666 | 0.74 | 0.363599 |
Target: 5'- uUCugcCCGAGGAaucgAGCGAcgaggccUUGAGGCCGCu -3' miRNA: 3'- uAGu--GGUUCCU----UCGUU-------AGCUCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 32163 | 0.71 | 0.511739 |
Target: 5'- --aGCCGAGGc-GCAG-CGAGGCCGa -3' miRNA: 3'- uagUGGUUCCuuCGUUaGCUCCGGCg -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 32440 | 0.71 | 0.480093 |
Target: 5'- uUCACCGgcuacAGGAAGCcgggCGAuGCCGCu -3' miRNA: 3'- uAGUGGU-----UCCUUCGuua-GCUcCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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