Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12496 | 5' | -53.5 | NC_003324.1 | + | 57038 | 0.68 | 0.667014 |
Target: 5'- --gGCUggGGAgcGGCGG-CGAGGgCGCg -3' miRNA: 3'- uagUGGuuCCU--UCGUUaGCUCCgGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 56799 | 0.66 | 0.785404 |
Target: 5'- --uGCC--GGAGGguGUCGAGcuuGCCGCu -3' miRNA: 3'- uagUGGuuCCUUCguUAGCUC---CGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 56295 | 0.72 | 0.459536 |
Target: 5'- uGUCGCCGccGAucgAGCGAUaggCGAGGCUGCc -3' miRNA: 3'- -UAGUGGUucCU---UCGUUA---GCUCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 56290 | 1.11 | 0.001005 |
Target: 5'- gAUCACCAAGGAAGCAAUCGAGGCCGCa -3' miRNA: 3'- -UAGUGGUUCCUUCGUUAGCUCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 56141 | 0.7 | 0.577331 |
Target: 5'- uUCGCCAGccuGGAAGC---CGAcGGCCGUg -3' miRNA: 3'- uAGUGGUU---CCUUCGuuaGCU-CCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 53478 | 0.66 | 0.785404 |
Target: 5'- -aCACCcgcgcGGAAGC---CGAGGCCGa -3' miRNA: 3'- uaGUGGuu---CCUUCGuuaGCUCCGGCg -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 49460 | 0.72 | 0.429605 |
Target: 5'- uUUGCCcGGGucGCuuUCGAGGUCGCc -3' miRNA: 3'- uAGUGGuUCCuuCGuuAGCUCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 49264 | 0.74 | 0.338735 |
Target: 5'- --gAUCGAGGAGcCGAUCGAGGCUGUc -3' miRNA: 3'- uagUGGUUCCUUcGUUAGCUCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 45920 | 0.68 | 0.655809 |
Target: 5'- -cCGCUucGGAAGCGAagcaagCGAcGCCGCa -3' miRNA: 3'- uaGUGGuuCCUUCGUUa-----GCUcCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 42127 | 0.67 | 0.743834 |
Target: 5'- cGUCGCCAaugccgccgcgGGGAucAGCAAcagaccauGGGCCGCg -3' miRNA: 3'- -UAGUGGU-----------UCCU--UCGUUagc-----UCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 40327 | 0.7 | 0.555192 |
Target: 5'- -gCACgCAAGGcGGCAGUCGAGcgggaGCCaGCa -3' miRNA: 3'- uaGUG-GUUCCuUCGUUAGCUC-----CGG-CG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 39956 | 0.71 | 0.511739 |
Target: 5'- gAUCGgCAAGGAAGCGgccgacaagaagGUCGAGGacaGCc -3' miRNA: 3'- -UAGUgGUUCCUUCGU------------UAGCUCCgg-CG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 39816 | 0.69 | 0.622103 |
Target: 5'- -aCACCAAGGccGCcauGUgGuGGCUGCa -3' miRNA: 3'- uaGUGGUUCCuuCGu--UAgCuCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 37651 | 0.66 | 0.764903 |
Target: 5'- gGUCGCCGAGGugguccGCAaugaacAUCGcaaGCCGCa -3' miRNA: 3'- -UAGUGGUUCCuu----CGU------UAGCuc-CGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 36714 | 0.67 | 0.722292 |
Target: 5'- uUCGCCGGGGAAaucuaugacccGCuAUCGcgcgggcacaAGGUCGCg -3' miRNA: 3'- uAGUGGUUCCUU-----------CGuUAGC----------UCCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 35733 | 0.69 | 0.622103 |
Target: 5'- -cCGCCAagaAGGAGGC---CGAGGCCa- -3' miRNA: 3'- uaGUGGU---UCCUUCGuuaGCUCCGGcg -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 34382 | 0.66 | 0.775231 |
Target: 5'- uUCGCCA----AGCGAUCGAGauaggcgaacucGCCGCc -3' miRNA: 3'- uAGUGGUuccuUCGUUAGCUC------------CGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 32557 | 0.67 | 0.711375 |
Target: 5'- gAUCGCCGAGGcugccggcgaAGGCGA-CGAgggcauGGCUGCc -3' miRNA: 3'- -UAGUGGUUCC----------UUCGUUaGCU------CCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 32440 | 0.71 | 0.480093 |
Target: 5'- uUCACCGgcuacAGGAAGCcgggCGAuGCCGCu -3' miRNA: 3'- uAGUGGU-----UCCUUCGuua-GCUcCGGCG- -5' |
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12496 | 5' | -53.5 | NC_003324.1 | + | 32163 | 0.71 | 0.511739 |
Target: 5'- --aGCCGAGGc-GCAG-CGAGGCCGa -3' miRNA: 3'- uagUGGUUCCuuCGUUaGCUCCGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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