Results 21 - 40 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12497 | 3' | -49.4 | NC_003324.1 | + | 30005 | 0.67 | 0.934014 |
Target: 5'- gGUGCACuc----UGGCGacGCGAUGCu -3' miRNA: 3'- aCGCGUGuuguaaACCGUa-CGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 33379 | 0.72 | 0.663663 |
Target: 5'- cGCGCGCAACuccggccacUGGCGgagaacgcuUGCGAUGa -3' miRNA: 3'- aCGCGUGUUGuaa------ACCGU---------ACGCUACg -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 33590 | 0.68 | 0.901916 |
Target: 5'- -uCGCACAuuACGUUUGGCG-GCGGg-- -3' miRNA: 3'- acGCGUGU--UGUAAACCGUaCGCUacg -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 33668 | 0.68 | 0.890049 |
Target: 5'- cUGCGCaaaGCAGCAgg-GGCgAUGUGAgaaaugacagauagcUGCa -3' miRNA: 3'- -ACGCG---UGUUGUaaaCCG-UACGCU---------------ACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 35403 | 0.66 | 0.939516 |
Target: 5'- cGCGCGCAugGUUUccucGCccuCGGUGCg -3' miRNA: 3'- aCGCGUGUugUAAAc---CGuacGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 35526 | 0.76 | 0.482125 |
Target: 5'- cUGCGCAcCGACGUUggUGGC-UGUGAucUGCg -3' miRNA: 3'- -ACGCGU-GUUGUAA--ACCGuACGCU--ACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 35692 | 0.68 | 0.897554 |
Target: 5'- cUGCGCGCAGCAgaagccgacaucGGCAagaaggaaGCcGAUGCg -3' miRNA: 3'- -ACGCGUGUUGUaaa---------CCGUa-------CG-CUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 37756 | 0.68 | 0.894588 |
Target: 5'- -aCGCACcguCAUgcGGCuUGCGAUGUu -3' miRNA: 3'- acGCGUGuu-GUAaaCCGuACGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 41624 | 0.69 | 0.853656 |
Target: 5'- cGCGCcgcugucguUAGCGUUUGGCAcGCGuUGUu -3' miRNA: 3'- aCGCGu--------GUUGUAAACCGUaCGCuACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 41679 | 0.72 | 0.698117 |
Target: 5'- cGCGCACuguGCcaacGGUA-GCGAUGCg -3' miRNA: 3'- aCGCGUGu--UGuaaaCCGUaCGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 41764 | 0.67 | 0.928207 |
Target: 5'- gGCGCAUcgcuACcgUUGGCAcagUGCGcgucgGCu -3' miRNA: 3'- aCGCGUGu---UGuaAACCGU---ACGCua---CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 42202 | 0.67 | 0.928207 |
Target: 5'- cGCG-GCGGCA-UUGGCgacgaGUGCGAaGCg -3' miRNA: 3'- aCGCgUGUUGUaAACCG-----UACGCUaCG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 42385 | 0.74 | 0.548431 |
Target: 5'- gGCGCcCGGCAcgUGGCAgGCGgcGCa -3' miRNA: 3'- aCGCGuGUUGUaaACCGUaCGCuaCG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 43036 | 0.66 | 0.944713 |
Target: 5'- cUGUGCACAugGCGUcaaGGCAUGgGAc-- -3' miRNA: 3'- -ACGCGUGU--UGUAaa-CCGUACgCUacg -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 44313 | 0.69 | 0.825945 |
Target: 5'- cGUGCACAGCGUU--GCGcGCGAUcaGCu -3' miRNA: 3'- aCGCGUGUUGUAAacCGUaCGCUA--CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 44512 | 0.68 | 0.87086 |
Target: 5'- -uCGgACGGCAcgc-GCAUGCGGUGCg -3' miRNA: 3'- acGCgUGUUGUaaacCGUACGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 44826 | 0.67 | 0.922092 |
Target: 5'- aGgGgGCGGCGUcaUUGGCGUGCccgcUGCu -3' miRNA: 3'- aCgCgUGUUGUA--AACCGUACGcu--ACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 47382 | 0.68 | 0.893839 |
Target: 5'- cGCGCGC------UGGUucgacaaAUGCGAUGCa -3' miRNA: 3'- aCGCGUGuuguaaACCG-------UACGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 48742 | 0.66 | 0.944713 |
Target: 5'- aUGCGCAgcCAGCGUcuccUGGCucAUGcCGAUGa -3' miRNA: 3'- -ACGCGU--GUUGUAa---ACCG--UAC-GCUACg -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 49411 | 0.65 | 0.957251 |
Target: 5'- cGcCGCACAGCAcgUGcagauacucacgauGUA-GCGAUGCg -3' miRNA: 3'- aC-GCGUGUUGUaaAC--------------CGUaCGCUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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