miRNA: 3'- aCGCGUG-UUGUAaAC-CGuACGCU----ACG- -5'
miRNA: 3'- aCGCGugUUGuaaacCGUACGCUaCG- -5'
miRNA: 3'- aCGCgUGUUGUaaaCCGU---ACGCUACG- -5'
miRNA: 3'- -ACGCGUGUUGUAAACCGUACGCUACG- -5'
miRNA: 3'- aCGCG-UGUUGUaa-ACCGu---ACGCUa-----CG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12497 | 3' | -49.4 | NC_003324.1 | + | 50647 | 0.71 | 0.742998 |
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Target: 5'- gGCGCACgAACAUcUGcGC-UGCGAcgguUGCg -3' miRNA: 3'- aCGCGUG-UUGUAaAC-CGuACGCU----ACG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 53700 | 0.67 | 0.908945 |
|
Target: 5'- gGCGCugGAC-----GCAUGCGAcGCc -3' miRNA: 3'- aCGCGugUUGuaaacCGUACGCUaCG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 54881 | 0.71 | 0.753941 |
|
Target: 5'- cGCGaACGGCGc--GGCGgucUGCGAUGCg -3' miRNA: 3'- aCGCgUGUUGUaaaCCGU---ACGCUACG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 56523 | 1.14 | 0.0017 |
|
Target: 5'- cUGCGCACAACAUUUGGCAUGCGAUGCg -3' miRNA: 3'- -ACGCGUGUUGUAAACCGUACGCUACG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 57338 | 0.66 | 0.949608 |
|
Target: 5'- aGCGCaAUAGCAggcUGGCcgcuUGCGAcccuugGCg -3' miRNA: 3'- aCGCG-UGUUGUaa-ACCGu---ACGCUa-----CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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