Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12497 | 3' | -49.4 | NC_003324.1 | + | 42202 | 0.67 | 0.928207 |
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Target: 5'- cGCG-GCGGCA-UUGGCgacgaGUGCGAaGCg -3' miRNA: 3'- aCGCgUGUUGUaAACCG-----UACGCUaCG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 7775 | 0.67 | 0.922092 |
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Target: 5'- cGCGC-CggUGUUUccggGGUcgGCGGUGUg -3' miRNA: 3'- aCGCGuGuuGUAAA----CCGuaCGCUACG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 19146 | 0.67 | 0.922092 |
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Target: 5'- aGCGCgACGACAaagGGCAaaUGgGcUGCa -3' miRNA: 3'- aCGCG-UGUUGUaaaCCGU--ACgCuACG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 22995 | 0.67 | 0.922092 |
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Target: 5'- cGCGCACucuucuuucGGCcgUaucGGCAUG-GAUGCu -3' miRNA: 3'- aCGCGUG---------UUGuaAa--CCGUACgCUACG- -5' |
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| 12497 | 3' | -49.4 | NC_003324.1 | + | 49411 | 0.65 | 0.957251 |
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Target: 5'- cGcCGCACAGCAcgUGcagauacucacgauGUA-GCGAUGCg -3' miRNA: 3'- aC-GCGUGUUGUaaAC--------------CGUaCGCUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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