Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12499 | 5' | -60 | NC_003324.1 | + | 1534 | 0.66 | 0.507132 |
Target: 5'- aUCcGCGCCGGauacgUCGcGCCGuuuCCUUUGCg -3' miRNA: 3'- gAGuUGCGGCC-----AGCaCGGC---GGGAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 30481 | 0.66 | 0.507132 |
Target: 5'- aUCGGCGCCGc-CGaGCagGCCCUCGg -3' miRNA: 3'- gAGUUGCGGCcaGCaCGg-CGGGAGCg -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 50673 | 0.66 | 0.497175 |
Target: 5'- gUCGAgGgCGG-CGUGCCaGCCCUUu- -3' miRNA: 3'- gAGUUgCgGCCaGCACGG-CGGGAGcg -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 50431 | 0.66 | 0.497175 |
Target: 5'- --aGGCGCUGGgCGUGgUGCagCUCGCa -3' miRNA: 3'- gagUUGCGGCCaGCACgGCGg-GAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 47653 | 0.66 | 0.487307 |
Target: 5'- gCUCGACGgCgagGGUCGauCgGCCCUCGUc -3' miRNA: 3'- -GAGUUGCgG---CCAGCacGgCGGGAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 40338 | 0.66 | 0.487307 |
Target: 5'- uUCGGCGCUGcagcaGUCuUGCCGgCUUUCGCu -3' miRNA: 3'- gAGUUGCGGC-----CAGcACGGC-GGGAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 38244 | 0.66 | 0.487307 |
Target: 5'- uUCAGCGCCGGaa-UGCCGa--UCGCc -3' miRNA: 3'- gAGUUGCGGCCagcACGGCgggAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 23405 | 0.66 | 0.477532 |
Target: 5'- gCUCGGCuuucucguugGCCGcGUCgGUGgCGCCCUgGUu -3' miRNA: 3'- -GAGUUG----------CGGC-CAG-CACgGCGGGAgCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 17816 | 0.66 | 0.477532 |
Target: 5'- cCUCAcgGCGCgcauuCGGg-GUGUCGCCCUUGa -3' miRNA: 3'- -GAGU--UGCG-----GCCagCACGGCGGGAGCg -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 11147 | 0.67 | 0.467855 |
Target: 5'- -gCuuCGCCGGUCuG-GCgGCCggCUCGCa -3' miRNA: 3'- gaGuuGCGGCCAG-CaCGgCGG--GAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 3358 | 0.67 | 0.467855 |
Target: 5'- gUUuAUGCCgacGGUUGccGCCGCCCUcCGCu -3' miRNA: 3'- gAGuUGCGG---CCAGCa-CGGCGGGA-GCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 30372 | 0.67 | 0.45828 |
Target: 5'- --uGGCGUCGcccUCGgccaUGCCGCCCUCGa -3' miRNA: 3'- gagUUGCGGCc--AGC----ACGGCGGGAGCg -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 23234 | 0.67 | 0.45828 |
Target: 5'- gUgGGCGCCGGcaagaCGUuuGCCGCCaUUGCa -3' miRNA: 3'- gAgUUGCGGCCa----GCA--CGGCGGgAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 38099 | 0.67 | 0.45828 |
Target: 5'- gUCGGCGCCGuGcUgGUGaCCGUUCUUGUg -3' miRNA: 3'- gAGUUGCGGC-C-AgCAC-GGCGGGAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 30059 | 0.67 | 0.45828 |
Target: 5'- aUCAcgcCGCCGGUCGcgacaccgGCCGCugcggcuuCCUCGa -3' miRNA: 3'- gAGUu--GCGGCCAGCa-------CGGCG--------GGAGCg -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 5451 | 0.67 | 0.454479 |
Target: 5'- aUCGACGCUGGgucaggcaggcggCGUcGcCCGCCCcaCGCu -3' miRNA: 3'- gAGUUGCGGCCa------------GCA-C-GGCGGGa-GCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 54150 | 0.67 | 0.44881 |
Target: 5'- gUCGA-GCCGGUCG-GCCGauuCCgcCGCa -3' miRNA: 3'- gAGUUgCGGCCAGCaCGGCg--GGa-GCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 24834 | 0.67 | 0.44881 |
Target: 5'- aUC-GCGCCgacaaGGUCG-GCCGUCUUgGCa -3' miRNA: 3'- gAGuUGCGG-----CCAGCaCGGCGGGAgCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 18492 | 0.67 | 0.43945 |
Target: 5'- -cCAGCGUCGccuUCGUGUgGuUCCUCGCa -3' miRNA: 3'- gaGUUGCGGCc--AGCACGgC-GGGAGCG- -5' |
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12499 | 5' | -60 | NC_003324.1 | + | 49602 | 0.67 | 0.430202 |
Target: 5'- cCUCGcCGCucgauaCGGcCGagagugccagaaUGCCGCCCUCGUu -3' miRNA: 3'- -GAGUuGCG------GCCaGC------------ACGGCGGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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