Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12529 | 3' | -51.5 | NC_003345.1 | + | 32404 | 0.66 | 0.928117 |
Target: 5'- uGUCGAacgucAGAACaCGGgGCggCGCGUCCa -3' miRNA: 3'- -CAGCU-----UCUUGaGUCgUGa-GCGUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 25836 | 0.66 | 0.928117 |
Target: 5'- cGUCGAAGuuCUCuGCGa--GCGUCCg -3' miRNA: 3'- -CAGCUUCuuGAGuCGUgagCGUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 73626 | 0.66 | 0.928117 |
Target: 5'- aGUCGucAGcGGCUCcGCGCcccaUCGCGUCCUc -3' miRNA: 3'- -CAGCu-UC-UUGAGuCGUG----AGCGUAGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 48537 | 0.66 | 0.933657 |
Target: 5'- gGUCGAuaaagguuccAGAcggcuACUCGGUACUCGUcacuaaCCCg -3' miRNA: 3'- -CAGCU----------UCU-----UGAGUCGUGAGCGua----GGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 27197 | 0.66 | 0.943906 |
Target: 5'- -gUGGAGGGCgCAGuUACUCGuCGUCCg -3' miRNA: 3'- caGCUUCUUGaGUC-GUGAGC-GUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 16194 | 0.66 | 0.943906 |
Target: 5'- aUCGAgccacGGAGCUucaacgacgacCGGCAUcggaGCAUCCCa -3' miRNA: 3'- cAGCU-----UCUUGA-----------GUCGUGag--CGUAGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 51136 | 0.66 | 0.943906 |
Target: 5'- -aCGAGGucgucaccGACUUGGaCGCUCGCGUUCa -3' miRNA: 3'- caGCUUC--------UUGAGUC-GUGAGCGUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 36966 | 0.66 | 0.948619 |
Target: 5'- -aCGAGGGuggCGGCuucuCUUGCGUUCCg -3' miRNA: 3'- caGCUUCUugaGUCGu---GAGCGUAGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 60107 | 0.66 | 0.948619 |
Target: 5'- uUgGGAGAGCUgAGC-CUgaGCGUUCCg -3' miRNA: 3'- cAgCUUCUUGAgUCGuGAg-CGUAGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 44130 | 0.66 | 0.948619 |
Target: 5'- -aCGAAGAcCUUguucGCACUCGUcuguguUCCCg -3' miRNA: 3'- caGCUUCUuGAGu---CGUGAGCGu-----AGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 74380 | 0.66 | 0.953062 |
Target: 5'- gGUCGaAAGAGCgUCAGCgGCUCaauuuCGUCCg -3' miRNA: 3'- -CAGC-UUCUUG-AGUCG-UGAGc----GUAGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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