Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12529 | 3' | -51.5 | NC_003345.1 | + | 46674 | 0.73 | 0.595277 |
Target: 5'- uUCGAGGAgucgaaccGC-CAGCACUCGUucgacguugaggaguAUCCCg -3' miRNA: 3'- cAGCUUCU--------UGaGUCGUGAGCG---------------UAGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 52566 | 0.68 | 0.873917 |
Target: 5'- aUCGAGGAugUuCAGCAcCUCGUugaCCu -3' miRNA: 3'- cAGCUUCUugA-GUCGU-GAGCGuagGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 38545 | 0.67 | 0.909829 |
Target: 5'- aGUUGAGGAACUUGGUAaucgaCGCAUCg- -3' miRNA: 3'- -CAGCUUCUUGAGUCGUga---GCGUAGgg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 32979 | 0.67 | 0.91432 |
Target: 5'- aGUUGAGGAACUUuugGGUguucuccgaguauaGCUCGUAgaagCCCu -3' miRNA: 3'- -CAGCUUCUUGAG---UCG--------------UGAGCGUa---GGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 40922 | 0.67 | 0.916203 |
Target: 5'- -cCGAAGGccacCUCGGUAaagCGCGUCCUu -3' miRNA: 3'- caGCUUCUu---GAGUCGUga-GCGUAGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 21720 | 0.67 | 0.916203 |
Target: 5'- -cCGgcGAACgucUCAGCAUcgagaaccuUCGCGUCCg -3' miRNA: 3'- caGCuuCUUG---AGUCGUG---------AGCGUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 32404 | 0.66 | 0.928117 |
Target: 5'- uGUCGAacgucAGAACaCGGgGCggCGCGUCCa -3' miRNA: 3'- -CAGCU-----UCUUGaGUCgUGa-GCGUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 25836 | 0.66 | 0.928117 |
Target: 5'- cGUCGAAGuuCUCuGCGa--GCGUCCg -3' miRNA: 3'- -CAGCUUCuuGAGuCGUgagCGUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 27197 | 0.66 | 0.943906 |
Target: 5'- -gUGGAGGGCgCAGuUACUCGuCGUCCg -3' miRNA: 3'- caGCUUCUUGaGUC-GUGAGC-GUAGGg -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 44130 | 0.66 | 0.948619 |
Target: 5'- -aCGAAGAcCUUguucGCACUCGUcuguguUCCCg -3' miRNA: 3'- caGCUUCUuGAGu---CGUGAGCGu-----AGGG- -5' |
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12529 | 3' | -51.5 | NC_003345.1 | + | 20165 | 1.11 | 0.002566 |
Target: 5'- gGUCGAAGAACUCAGCACUCGCAUCCCc -3' miRNA: 3'- -CAGCUUCUUGAGUCGUGAGCGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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