Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12581 | 3' | -47.5 | NC_003345.1 | + | 75706 | 0.72 | 0.914247 |
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Target: 5'- ---uGCGGCGGAggcuguucUCGGUGGGggagaaGCGGc -3' miRNA: 3'- cuuuUGCCGCCUa-------AGCCAUUCa-----UGCC- -5' |
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| 12581 | 3' | -47.5 | NC_003345.1 | + | 64671 | 0.73 | 0.893719 |
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Target: 5'- uGAGAGC-GCGGGUUCGGUAcGUAUc- -3' miRNA: 3'- -CUUUUGcCGCCUAAGCCAUuCAUGcc -5' |
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| 12581 | 3' | -47.5 | NC_003345.1 | + | 55758 | 0.73 | 0.886321 |
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Target: 5'- --uGACGGCGGAUaCGGUcacggAAGUauucACGGc -3' miRNA: 3'- cuuUUGCCGCCUAaGCCA-----UUCA----UGCC- -5' |
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| 12581 | 3' | -47.5 | NC_003345.1 | + | 768 | 0.73 | 0.878654 |
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Target: 5'- -cGAACGGuCGGGggCgGGUAAGUACGu -3' miRNA: 3'- cuUUUGCC-GCCUaaG-CCAUUCAUGCc -5' |
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| 12581 | 3' | -47.5 | NC_003345.1 | + | 77575 | 0.77 | 0.67113 |
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Target: 5'- uGAAAACGGCGGAUUCGGU-------- -3' miRNA: 3'- -CUUUUGCCGCCUAAGCCAuucaugcc -5' |
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| 12581 | 3' | -47.5 | NC_003345.1 | + | 2309 | 0.87 | 0.239443 |
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Target: 5'- gGAAAACGGCGGAUUCGGUGugaAGccccucGCGGa -3' miRNA: 3'- -CUUUUGCCGCCUAAGCCAU---UCa-----UGCC- -5' |
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| 12581 | 3' | -47.5 | NC_003345.1 | + | 288 | 1.11 | 0.008704 |
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Target: 5'- uGAAAACGGCGGAUUCGGUAAGUACGGc -3' miRNA: 3'- -CUUUUGCCGCCUAAGCCAUUCAUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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