Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12617 | 3' | -56.6 | NC_003345.1 | + | 50102 | 0.67 | 0.706454 |
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Target: 5'- gCGGUgGAGcuauuauCGuuGUCAGCC-CCGg -3' miRNA: 3'- aGCCAgCUUu------GCggCAGUCGGaGGCg -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 21239 | 0.67 | 0.706454 |
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Target: 5'- uUCcGUCGAuuaccuccACGUCGUCAGCgUCCa- -3' miRNA: 3'- -AGcCAGCUu-------UGCGGCAGUCGgAGGcg -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 43021 | 0.67 | 0.726909 |
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Target: 5'- -aGGUCGAuAC-CUGUCAG-CUCaCGCu -3' miRNA: 3'- agCCAGCUuUGcGGCAGUCgGAG-GCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 54043 | 0.66 | 0.765726 |
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Target: 5'- gCGG-CGAGAacCGCgaGUCGGacgaaaaCCUCCGCg -3' miRNA: 3'- aGCCaGCUUU--GCGg-CAGUC-------GGAGGCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 32773 | 0.66 | 0.785874 |
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Target: 5'- cCGGUgCGAGAgaGCgGUCGGCgUugacaCCGCg -3' miRNA: 3'- aGCCA-GCUUUg-CGgCAGUCGgA-----GGCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 21340 | 0.76 | 0.241653 |
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Target: 5'- uUCGGUCGGAGCGCCGUU-GUCgUCgGUg -3' miRNA: 3'- -AGCCAGCUUUGCGGCAGuCGG-AGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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