Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12617 | 3' | -56.6 | NC_003345.1 | + | 71025 | 0.69 | 0.591229 |
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Target: 5'- gUCGGUCGAAACcggcuucggGCUGUCGuCgUCCGg -3' miRNA: 3'- -AGCCAGCUUUG---------CGGCAGUcGgAGGCg -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 72393 | 0.67 | 0.71672 |
|
Target: 5'- cUCGGUCGGGagaauACGCgGUCGGaa-CUGCu -3' miRNA: 3'- -AGCCAGCUU-----UGCGgCAGUCggaGGCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 73067 | 0.66 | 0.766699 |
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Target: 5'- uUCGGUgGGAACGCCGcgagaaCGGUCgCCa- -3' miRNA: 3'- -AGCCAgCUUUGCGGCa-----GUCGGaGGcg -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 73613 | 0.66 | 0.766699 |
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Target: 5'- -aGGcCGuAGGaguaGUCGUCAGCggCUCCGCg -3' miRNA: 3'- agCCaGC-UUUg---CGGCAGUCG--GAGGCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 75574 | 0.7 | 0.543523 |
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Target: 5'- -gGGUCGAAGCucggcucaucGCCGUCuucucccccaccgagAacaGCCUCCGCc -3' miRNA: 3'- agCCAGCUUUG----------CGGCAG---------------U---CGGAGGCG- -5' |
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| 12617 | 3' | -56.6 | NC_003345.1 | + | 77013 | 0.68 | 0.643803 |
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Target: 5'- cUCGGUCGggGaGCUgGUCgAGCCaaUCCGg -3' miRNA: 3'- -AGCCAGCuuUgCGG-CAG-UCGG--AGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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